miR-195-5p Regulates Hair Follicle Inductivity of Dermal Papilla Cells by Suppressing Wnt/β -Catenin Activation

BioMed Research International
Ningxia ZhuChang-min Lin

Abstract

Dermal papilla (DP) cells play a vital role in hair follicle (HF) development and postnatal hair cycling. However, the abilities are lost on further culture. Recent studies have demonstrated significant influences of posttranscriptional regulation by microRNA (miRNA) on HF development. The current study aims to investigate how miRNAs regulate Wnt/β-catenin to control HF inductivity of DP cells by performing microarray analysis in early- and late-passage DP cells and transfecting with miRNAs inhibitor or mimic. Results showed early-passage DP cells strongly expressed miRNAs related to inhibition of noncanonical Wnt pathways. In late-passage DP cells, miRNAs capable of inhibiting the canonical Wnt/β-catenin pathway were upregulated, in addition to the miRNAs targeting the noncanonical Wnt pathway. Moreover, we verified that β-catenin expression was downregulated by miR-195-5p overexpression in dose manner. Meanwhile LRP6 expression was downregulated in both protein and mRNA as well as the genes involved in the hair inductivity of DP cells. These results suggest that the appearance of miRNAs that suppress the Wnt/β-catenin pathway may be responsible for the loss of ability of DP cells in culture and miR-195-5p is the potential key...Continue Reading

References

Oct 1, 1996·Dermatologic Clinics·C A Jahoda, A J Reynolds
Nov 13, 1999·Nature Cell Biology·V A BotchkarevR Paus
Feb 20, 2002·The Journal of Investigative Dermatology·Vladimir A BotchkarevBarbara A Gilchrest
May 23, 2002·Developmental Cell·Thomas AndlSarah E Millar
Feb 23, 2007·Nature·Elaine Fuchs
Dec 31, 2008·Proceedings of the National Academy of Sciences of the United States of America·Rui YiElaine Fuchs
Jul 22, 2009·Developmental Cell·Bryan T MacDonaldXi He
Apr 24, 2010·Developmental Cell·David Enshell-SeijffersBruce A Morgan
Jun 5, 2010·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Andrei N MardaryevNatalia V Botchkareva
May 25, 2012·Journal of Cell Science·Manabu OhyamaMasayuki Amagai
Jun 20, 2013·International Journal of Medical Sciences·Yi-Zhan XingTian Yang
Oct 16, 2013·The Journal of Investigative Dermatology·Seung H ShinYoung K Sung
Oct 22, 2013·Nature·Maria Carolina FlorianHartmut Geiger
Nov 16, 2013·Cell Death & Disease·I AmelioE Candi
Dec 7, 2013·Developmental Biology·Su-Yi TsaiMichael Rendl
Oct 7, 2014·Biochemical and Biophysical Research Communications·Chang-Min LinYu Li
Nov 11, 2014·Journal of Tissue Engineering·Peipei ZhangKacey G Marra
Nov 26, 2014·The Journal of Cell Biology·Mohammed I AhmedNatalia V Botchkareva
Apr 7, 2015·Cellular Signalling·Jia L SongErica Selva
May 9, 2015·Advanced Drug Delivery Reviews·Kyle J MillerHoward Levinson
Feb 26, 2016·Experimental Dermatology·Mi Hee KwackMoon Kyu Kim

❮ Previous
Next ❯

Methods Mentioned

BETA
electrophoresis
transfection

Software Mentioned

GenePix Pro
TargetScan
MEV
miRanda
Target Scan

Related Concepts

Related Feeds

Adherens Junctions

An adherens junction is defined as a cell junction whose cytoplasmic face is linked to the actin cytoskeleton. They can appear as bands encircling the cell (zonula adherens) or as spots of attachment to the extracellular matrix (adhesion plaques). Adherens junctions uniquely disassemble in uterine epithelial cells to allow the blastocyst to penetrate between epithelial cells. Discover the latest research on adherens junctions here.

Cadherins and Catenins

Cadherins (named for "calcium-dependent adhesion") are a type of cell adhesion molecule (CAM) that is important in the formation of adherens junctions to bind cells with each other. Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells: alpha-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. Discover the latest research on cadherins and catenins here.