miR-195-5p/NOTCH2-mediated EMT modulates IL-4 secretion in colorectal cancer to affect M2-like TAM polarization

Journal of Hematology & Oncology
Xiaobin LinBin Xiong

Abstract

Tumor microenvironment (TME) is a complex environment containing tumor cells, tumor-associated macrophages (TAMs), interstitial cells, and non-cellular components. Epithelial-mesenchymal transition (EMT), as a major actor in cancer tumorigenicity and metastasis, was involved in the interaction between TAMs and tumor cells. However, the potential mechanisms of EMT and how EMT-programmed tumor cells affect M2-like TAMs still need further exploration. An integrated analysis of nine CRC miRNA expression datasets was performed. Functional assays, including the EdU, clone formation, wound healing, and transwell assays, were used to determine the anticancer role of miR-195-5p in human CRC progression. Furthermore, RNA immunoprecipitation, RNA decay, and dual-luciferase reporter assays were used to determine the mechanism of miR-195-p CRC progression. Then co-culture, migration, and ELISA assays were applied to determine the role of miR-195-5p in macrophage recruitment and alternative polarization. Xenograft mouse models were used to determine the role of miR-195-5p in CRC tumorigenicity and TAM polarization in vivo. An integrated analysis confirmed that miR-195-5p was significantly downregulated in CRC tissues, and patients with a low...Continue Reading

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Methods Mentioned

BETA
transfection
immunoprecipitation
PCR
electrophoresis
xenograft
fluorescence microscopy
immunoprecipitation assay
ELISA
RIP
Flow cytometry

Software Mentioned

SPSS
Gene Set Enrichment Analysis ( GSEA
GSEA

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