MiR-200-3p Is Potentially Involved in Cell Cycle Arrest by Regulating Cyclin A during Aestivation in Apostichopus japonicus

Cells
Shanshan WangKenneth B Storey

Abstract

The sea cucumber (Apostichopus japonicus) has become a good model organism for studying environmentally induced aestivation in marine invertebrates. We hypothesized that mechanisms that arrest energy-expensive cell cycle activity would contribute significantly to establishing the hypometabolic state during aestivation. Cyclin A is a core and particularly interesting cell cycle regulator that functions in both the S phase and in mitosis. In the present study, negative relationships between miR-200-3p and AjCA expressions were detected at both the transcriptional and the translational levels during aestivation in A. japonicus. Dual-luciferase reporter assays confirmed the targeted location of the miR-200-3p binding site within the AjCA gene transcript. Furthermore, gain- and loss-of-function experiments were conducted in vivo with sea cucumbers to verify the interaction between miR-200-3p and AjCA in intestine tissue by qRT-PCR and Western blotting. The results show that the overexpression of miR-200-3p mimics suppressed AjCA transcript levels and translated protein production, whereas transfection with a miR-200-3p inhibitor enhanced both AjCA mRNA and AjCA protein in A. japonicus intestine. Our findings suggested a potential me...Continue Reading

References

Feb 16, 2002·Cellular and Molecular Life Sciences : CMLS·A J Obaya, J M Sedivy
Mar 10, 2004·Biological Reviews of the Cambridge Philosophical Society·Kenneth B Storey, Janet M Storey
May 10, 2007·The Journal of Experimental Biology·Kenneth B Storey, Janet M Storey
May 16, 2008·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Fei GaoDonovan P German
Jun 2, 2010·Current Genomics·Kyle K Biggar, Kenneth B Storey
Dec 24, 2010·Journal of Molecular Cell Biology·Kyle K Biggar, Kenneth B Storey
Nov 23, 2013·Cancer Letters·Xiangling FengYaguang Xi
Apr 10, 2014·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Ye ZhaoMuyan Chen
Jun 18, 2014·International Journal of Medical Sciences·Peng ZhangJishuai Zhang
Jul 5, 2016·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Muyan ChenTianming Wang
Oct 7, 2016·Science·Arun KanakkantharaJan M van Deursen
Jun 1, 2017·Nature Reviews. Molecular Cell Biology·Thomas D Pollard
Dec 6, 2017·Journal of Molecular Cell Biology·Kyle K Biggar, Kenneth B Storey
May 29, 2018·Journal of Experimental Zoology. Part A, Ecological and Integrative Physiology·Shasha WangTianming Wang
Nov 6, 2018·The Journal of Endocrinology·Kate E LinesRajesh V Thakker

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Citations

May 20, 2020·The Journal of Experimental Biology·Hanane Hadj-Moussa, Kenneth B Storey

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Methods Mentioned

BETA
environmental stress
PCR
transfection
Assay

Software Mentioned

DNAStar
Image
Pro Plus
Miranda
TargetScan
SPSS

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