miR-200a-5p augments cardiomyocyte hypertrophy induced by glucose metabolism disorder via the regulation of selenoproteins

Journal of Cellular Physiology
Tianshu YangShiwen Xu

Abstract

Selenium and selenoproteins are identified as potential determinants in pathological cellular hypertrophy. Cardiomyocytes hypertrophy is a compensatory form of heart disease characterized by increased size of cardiomyocytes. However, the link between cardiac hypertrophy and Se-specific microRNA (miRNA) remains to be characterized. In the current study, we established a miR-200a-5p mimic and an inhibitor cardiomyocytes model. Cardiomyocytes hypertrophy was induced in the miR-200a-5p mimic group. Hence, we detected the glucose level of cardiomyocytes to estimate the cellular glucose uptake. The effect of miR-200a-5p overexpression and the low expression on 25 selenoproteins mRNA levels was further explored using reverse transcription polymerase chain reaction. Overexpression of miR-200a-5p elevated glucose uptake and Txnrd2, 3 expression and reduced Sepp1, Seln, Selt, and Sep15 expression in cardiomyocytes. Contrary results were observed in cardiomyocytes with the knockdown of miR-200a-5p. We next assessed glucose metabolism-related genes in cardiomyocytes. The results showed that miR-200a-5p had a negative correlation with insulin-like growth factor gene-1, insulin-like growth factor binding protein (IGFBP)1, IGFBP2, IGFBP3, IGF...Continue Reading

References

Feb 1, 1995·Endocrine Reviews·J I Jones, D R Clemmons
Jan 1, 1995·Progress in Growth Factor Research·B TönshoffO Mehls
Dec 5, 1998·The Journal of Biological Chemistry·D C ThurmondJ E Pessin
Aug 30, 2000·Lancet·M P Rayman
Sep 2, 2000·Diabetes·M T GuillamB Thorens
Mar 14, 2001·Circulation·P H Sugden
Jan 14, 2003·Annual Review of Physiology·N Frey, E N Olson
Feb 6, 2003·The British Journal of Nutrition·I Stuart Wood, Paul Trayhurn
May 20, 2003·Nature·Pere PuigserverBruce M Spiegelman
Sep 25, 2004·Journal of Cellular Physiology·Maria L MachedaJames D Best
Nov 30, 2005·Cellular and Molecular Life Sciences : CMLS·M RederstorffA Lescure
Dec 2, 2005·The Journal of Biological Chemistry·Andrew D FergusonJohann Deisenhofer
Oct 13, 2006·Antioxidants & Redox Signaling·Gabriela M KusterWilson S Colucci
Oct 19, 2006·Current Opinion in Cell Biology·Reuben J Shaw
Nov 17, 2006·Proceedings of the National Academy of Sciences of the United States of America·Eva van RooijEric N Olson
May 15, 2007·Journal of Molecular and Cellular Cardiology·Mariko TatsuguchiDa-Zhi Wang
Jan 17, 2008·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Luca GrumolatoYoussef Anouar
Oct 8, 2009·Cell Metabolism·Kim LohTony Tiganis
Dec 17, 2009·Diabetes/metabolism Research and Reviews·Biao FengSubrata Chakrabarti
Nov 3, 2010·Cell Metabolism·Hirofumi MisuShuichi Kaneko
May 31, 2011·Archives of Biochemistry and Biophysics·FuKun W HoffmannPeter R Hoffmann
Aug 13, 2013·Molecular Nutrition & Food Research·Anabel Maciel-DominguezJohn Hesketh
Feb 19, 2017·Journal of Inorganic Biochemistry·Jie YangZiwei Zhang
May 2, 2017·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Marwa TarekIman F Montasser
May 17, 2018·Metallomics : Integrated Biometal Science·Zhepeng SunShu Li

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Citations

Oct 17, 2019·Human Gene Therapy. Clinical Development·Kefeng Cai, Huiqin Chen
Nov 22, 2019·Biological Trace Element Research·Yilei ZhangShiwen Xu
Aug 8, 2020·Frontiers in Physiology·Carolina Victoria Cruz JunhoMarcela Sorelli Carneiro-Ramos
Nov 30, 2019·Oxidative Medicine and Cellular Longevity·Xiaoping HuLuocheng Li
Aug 10, 2021·Frontiers in Cardiovascular Medicine·Hengzhi DuChen Chen

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