miR-200c regulates the proliferation, apoptosis and invasion of gastric carcinoma cells through the downregulation of EDNRA expression
Abstract
This study aimed to investigate the regulatory effects of microRNA (miR)‑200c on the proliferation, apoptosis and invasion of gastric carcinoma cells and to elucidate the underlying mechanisms involving the possible role of endothelin receptor A (EDNRA). The expression levels of miR‑200c and EDNRA in the gastric carcinoma cell lines, BGC‑823, SGC‑7901 and HGC‑27, and in GES‑1 normal gastric cells were evaluated by RT‑PCR and western blot analysis. The gastric carcinoma cells, particularly the BGC‑823 cells, expressed significantly lower levels of miR‑200c than the normal gastric cells (P<0.01). Thus, the BGC‑823 cells were employed as model cells. In comparison to the normal gastric cells, EDNRA was overexpressed in the gastric carcinoma cells (P<0.01). Following the transfection of the gastric carcinoma cells with miR‑200c mimics, or negative control vector (miR‑200c NC), or with siRNA targeting EDNRA (siRNA EDNRA) or negatvie control siRNA (siRNA NC), the expression levels were assessed again by RT‑PCR and western blot analysis. The successful transfection of miR‑200c mimics was observed and this markedly elevated the expression of miR‑200c (P<0.01). The transfection of miR‑200c mimics or siRNA EDNRA notably decreased the EDN...Continue Reading
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Apoptosis
Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis