miR-203 inhibits ovarian tumor metastasis by targeting BIRC5 and attenuating the TGFβ pathway

Journal of Experimental & Clinical Cancer Research : CR
Baojin WangJunming Yue

Abstract

We previously reported that miR-203 functions as a tumor suppressor in ovarian cancer cells by directly targeting transcription factor Snai2 and inhibiting epithelial to mesenchymal transition (EMT), whereas BIRC5/survivin promotes EMT. In this study, we tested our hypothesis that miR-203 inhibits ovarian tumor metastasis by suppressing EMT through targeting BIRC5, using an orthotopic ovarian cancer mouse model. We overexpressed miR-203 in ovarian cancer SKOV3 and OVCAR3 cells using a lentiviral vector and examined cell migration and invasion using transwell plates. The small molecule inhibitor, YM155, was used to inhibit survivin expression. miR-203-expressing and control SKOV3 cells were intrabursally injected into immunocompromised NSG female mice. Primary tumors in ovaries and metastatic tumors were collected to determine the expression of survivin and EMT markers using Western blot and immunostaining. Overexpression of miR-203 inhibits EMT by targeting BIRC5 in ovarian cancer SKOV3 and OVCAR3 cells. miR-203 expression enhances the ability of the survivin inhibitor YM155 to reduce tumor cell migration and invasion in vitro. We further showed that miR-203 expression attenuated the TGFβ pathway in both SKOV3 and OVCAR3 cells....Continue Reading

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Citations

Aug 24, 2019·Journal of Experimental & Clinical Cancer Research : CR·Fengzhi LiXiang Ling
Jul 10, 2019·Current Drug Targets·Seyed Mostafa ParizadehAmir Avan
Apr 25, 2020·Expert Review of Anticancer Therapy·Huilin Zhang, Bingjian Lu
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Apr 21, 2021·International Journal of Biological Macromolecules·Shufang LiYi Zou

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Methods Mentioned

BETA
dissecting microscopy

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