miR-204 downregulates EphB2 in aging mouse hippocampal neurons

Aging Cell
Chand Parvez Danka MohammedKeetae Kim

Abstract

Hippocampal synaptic function and plasticity deteriorate with age, often resulting in learning and memory deficits. As MicroRNAs (miRNAs) are important regulators of neuronal protein expression, we examined whether miRNAs may contribute to this age-associated decline in hippocampal function. We first compared the small RNA transcriptome of hippocampal tissues from young and old mice. Among 269 hippocampal miRNAs, 80 were differentially expressed (≥ twofold) among the age groups. We focused on 36 miRNAs upregulated in the old mice compared with those in the young mice. The potential targets of these 36 miRNAs included 11 critical Eph/Ephrin synaptic signaling components. The expression levels of several genes in the Eph/Ephrin pathway, including EphB2, were significantly downregulated in the aged hippocampus. EphB2 is a known regulator of synaptic plasticity in hippocampal neurons, in part by regulating the surface expression of the NMDA receptor NR1 subunit. We found that EphB2 is a direct target of miR-204 among miRNAs that were upregulated with age. The transfection of primary hippocampal neurons with a miR-204 mimic suppressed both EphB2 mRNA and protein expression and reduced the surface expression of NR1. Transfection of m...Continue Reading

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Citations

May 19, 2017·Nature Reviews. Neuroscience·Akiko SatohLeonard Guarente
Jan 26, 2017·Mechanisms of Ageing and Development·Chand Parvez Danka MohammedKeetae Kim
May 11, 2017·Journal of Neurochemistry·Simona Federica SpampinatoMaria Angela Sortino
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Datasets Mentioned

BETA
GSE74796

Methods Mentioned

BETA
PCR
transfection
light microscopy
RNA seq

Software Mentioned

Excel
nih imagej
TargetScan

Related Concepts

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