miR-204 functions as a tumor suppressor gene, at least partly by suppressing CYP27A1 in glioblastoma

Oncology Letters
Jun XinLi-Qiang Tian

Abstract

Gliomas are the most common type of malignant primary brain tumors in adults and exhibit a spectrum of aberrantly aggressive phenotypes. Despite advances in treatments during past decades, prognosis of the disease remains poor, with a median survival time of 12-14 months. Future studies on the molecular mechanism of the disease may provide the theoretical basis to identify new targets for effective therapies. The present study revealed that in glioblastoma cells, the overexpression of cytochrome P450, family 27, subfamily A, polypeptide 1 (CYP27A1) promoted proliferation, while silencing of CYP27A1 inhibited proliferation, without affecting migration and invasion. CYP27A1 protein was upregulated in glioblastoma tissues, indicating that CYP27A1 is an oncogene. The downregulation of specific microRNAs (miRNA) may contribute to the upregulation of oncogenes in glioblastoma. A common strategy was used to predict target miRNAs of CPY27A1 using the miRanda algorithm. miR-211 and miR-204 could target the 3'untranslated region of CPY27A1 mRNA. Additional studies confirmed that the overexpression of miR-204 inhibited CPY27A1 expression in glioblastoma cells. Finally, it was identified that miR-204 was downregulated in glioblastoma and t...Continue Reading

References

May 26, 1992·Biochemistry·D W Russell, K D Setchell
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Jul 19, 2005·Cancer Research·Jennifer A ChanKenneth S Kosik
Sep 19, 2007·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Damien Matusiak, Richard V Benya
Feb 14, 2008·Cell Research·Shuomin ZhuYin-Yuan Mo
Mar 15, 2011·International Journal of Cancer. Journal International Du Cancer·T K H ChungY F Wong
Nov 16, 2012·Cell Death & Disease·A SacconiG Blandino
Oct 18, 2013·The New England Journal of Medicine·Sunit Das, Philip A Marsden
Sep 2, 2016·Science Translational Medicine·Marie AllenBengt Westermark
Jan 10, 2018·CA: a Cancer Journal for Clinicians·Rebecca L SiegelAhmedin Jemal

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Citations

Jul 16, 2019·Journal of Environmental Science and Health. Part C, Environmental Carcinogenesis & Ecotoxicology Reviews·Dongying LiBaitang Ning
Jul 25, 2021·Cancers·Giuseppe FiorentinoLuca Marchetti

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Methods Mentioned

BETA
Protein Assay
PCR
transfection

Software Mentioned

miRanda
ImageJ
GraphPad Prism
GraphPad

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