miR-205-5p inhibits cell migration and invasion in prostatic carcinoma by targeting ZEB1

Oncology Letters
Lianpeng Li, Shouqiang Li

Abstract

Many studies have demonstrated that miRNAs have influence on tumorigenesis and progression of human cancers, including invasion and migration. Thus, the role of miR-205/ZEB1 axis for the migration and invasion of prostate cancer cells was explored in the present study. The miR-205-5p and zinc finger E-box binding homeobox 1 (ZEB1) mRNA expression levels were observed in prostate cancer tissues or cell lines via reverse transcription-quantitative PCR (RT-qPCR), and the protein level of ZEB1 was measured by western blotting. Dual-Luciferase Reporter Assay was used to verify the relationship between miR-205-5p and ZEB1. In addition, cell migration and invasion was measured by Transwell assay. The results revealed that, compared with the control, downregulation of miR-205-5p was detected in prostate cancer tissues and cell lines, and miR-205-5p overexpression was found to inhibit cell migration and invasion. Moreover, miR-205-5p was confirmed to directly target ZEB1 in prostate cancer. Importantly, ZEB1 was identified to weaken the inhibitory effect of miR-205-5p in prostate cancer. In conclusion, miR-205-5p inhibited cell migration and invasion in prostatic carcinoma by targeting ZEB1 and miR-205-5p/ZEB1 axis shows potential to be...Continue Reading

References

Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Jul 13, 2005·Cell·Carlo M Croce, George A Calin
Jan 30, 2008·Brain Pathology·Xuhang LiuZissimos Mourelatos
Nov 10, 2009·Bioinformatics·Stefano VoliniaCarlo M Croce
Apr 25, 2013·International Journal of Oncology·Berlinda VerdoodtAlireza Mirmohammadsadegh
Jan 24, 2015·Pharmacology & Therapeutics·Zufan Debebe, W Kimryn Rathmell
Apr 30, 2015·Japanese Journal of Clinical Oncology·Xianglun SunJie Zhang
May 10, 2017·Journal of Ovarian Research·Jun WeiHelen H Zhou
May 14, 2017·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Baoqi DuanZhaoqing Xi
Jun 28, 2017·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Hui Pang, Xiuying Yue
Jul 21, 2017·International Braz J Urol : Official Journal of the Brazilian Society of Urology·Zhi-Gang YangYing-Xin Guo
Sep 14, 2017·Journal of Cancer·Xian-Lu SongZi-Qing Wu

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Citations

Dec 22, 2019·Journal of Cellular and Molecular Medicine·Maria OltraJorge M Barcia

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Methods Mentioned

BETA
Assay

Software Mentioned

TargetScan
GraphPad Prism
SPSS

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