MiR-205 mediated APC regulation contributes to pancreatic cancer cell proliferation

World Journal of Gastroenterology : WJG
Rui-Feng QinJia-Dong Xue

Abstract

Pancreatic cancer is a deadly malignancy with aggressive properties. MicroRNAs (miRNAs) participate in the pathogenesis of a variety of diseases and molecular processes by targeting functional mRNAs. Nevertheless, the regulatory role of miRNAs in signaling pathways involved in pancreatic cancer remains largely unknown. To explore the molecular regulation involved in pancreatic cancer and potential mechanisms of miR-205. Microarray analysis was performed to investigate the expression profile of miRNAs in pancreatic cancer. Expression of miR-205 was validated by qRT-PCR. Target prediction and functional enrichment analysis were employed to seek potential target genes of miR-205 and potential functions of these genes. The target binding of miR-205 and adenomatous polyposis coli (APC) was validated by luciferase reporter assay. APC protein expression in pancreatic cancer was validated by qRT-PCR and Western blot. Proliferation was evaluated by MTT and colony formation assays. A large number of miRNAs with altered expression were identified in pancreatic cancer. MiR-205 was significantly up-regulated. APC was found to be a validated target of miR-205 and down-regulated in pancreatic cancer. Proliferation experiments showed that miR-...Continue Reading

References

Apr 24, 2013·CMAJ : Canadian Medical Association Journal = Journal De L'Association Medicale Canadienne·Zaheer S Kanji, Steven Gallinger
Sep 16, 2014·Oncology Reports·Siliang WangMeiyue Tang
Nov 8, 2014·Nucleic Acids Research·Nathan Wong, Xiaowei Wang
Apr 1, 2015·Nature Reviews. Clinical Oncology·Ignacio Garrido-Laguna, Manuel Hidalgo
Aug 13, 2015·ELife·Vikram AgarwalDavid P Bartel
Oct 21, 2015·Genomics Data·Lidia DaimielAlberto Dávalos
Jan 23, 2016·Biochimica Et Biophysica Acta·Marco FalascaIlaria Casari
Feb 18, 2017·Nature Reviews. Drug Discovery·Rajesha Rupaimoole, Frank J Slack
Apr 17, 2017·Cancer Letters·Tina Catela IvkovicYvonne Ceder
Aug 2, 2017·Cell·Jae-Seok RoeChristopher R Vakoc
Sep 13, 2017·Cancers·Nicola GaianigoCarmine Carbone
Oct 22, 2017·The Journal of Pharmacology and Experimental Therapeutics·Anita ThyagarajanRavi Prakash Sahu
Nov 7, 2017·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Aditi VarshneyVirander S Chauhan
Mar 14, 2018·Developmental Cell·Kenyi Saito-DiazEthan Lee

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Citations

Aug 29, 2020·Cells·Neeraj ChauhanMurali M Yallapu
Jun 1, 2021·Epigenetics : Official Journal of the DNA Methylation Society·I Gede Putu SupadmanabaElisa Giovannetti
Jun 3, 2021·Cancers·Iram FatimaVishal Chandra
Jul 31, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Mohadeseh FathiMohammad Taheri

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Methods Mentioned

BETA
transfection
Assay
Protein Assay

Software Mentioned

KOBAS
PrimerBank
targetScan
R script

Related Concepts

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Adenomatous Polyposis Coli

Adenomatous polyposis coli is a protein encoded by the APC gene and acts as a tumor suppressor. Discover the latest research on adenomatous polyposis coli here.

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