MiR-21 is enriched in the RNA-induced silencing complex and targets COL4A1 in human granulosa cell lines

Reproductive Sciences
Yuri MaseToshihiro Takizawa

Abstract

MicroRNAs (miRNAs) are noncoding small RNAs that play important roles in a variety of physiological and pathological events. In this study, we performed large-scale profiling of EIF2C2-bound miRNAs in 3 human granulosa-derived cell lines (ie, KGN, HSOGT, and GC1a) by high-throughput sequencing and found that miR-21 accounted for more than 80% of EIF2C2-bound miRNAs, suggesting that it was enriched in the RNA-induced silencing complex (RISC) and played a functional role in human granulosa cell (GC) lines. We also found high expression levels of miR-21 in primary human GCs. Assuming that miR-21 target mRNAs are enriched in RISC, we performed cDNA cloning of EIF2C2-bound mRNAs in KGN cells. We identified COL4A1 mRNA as a miR-21 target in the GC lines. These data suggest that miR-21 is involved in the regulation of the synthesis of COL4A1, a component of the basement membrane surrounding the GC layer and granulosa-embedded extracellular structure.

References

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Citations

May 14, 2014·BioMed Research International·Kouji BannoDaisuke Aoki
Aug 27, 2014·Gynécologie, obstétrique & fertilité·E ScaliciS Hamamah
Dec 15, 2015·Stem Cells International·Irma Virant-KlunThomas Skutella
Aug 26, 2014·Journal of the American Society of Nephrology : JASN·Jennifer Y LaiMarkus Bitzer
Apr 15, 2020·Human Fertility : Journal of the British Fertility Society·Imen BelguithLeila Ammar Keskes
May 1, 2020·Journal of Immunology Research·Xinran LiXiafei Fu
May 17, 2021·Reproductive Biology and Endocrinology : RB&E·Haroon Latif KhanEnder Yalcinkaya Kalyan

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Methods Mentioned

BETA
electrophoresis
PCR
Assay
transfection

Software Mentioned

MicroCosm
Multi Gauge

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