Abstract
In this study, we investigated whether the metabolic alteration of cancer-associated fibroblasts (CAFs) occurs via miR-21 remodeling and the effect of this alteration on pancreatic cancer cells. CAFs and normal fibroblasts (NFs) were isolated and cultured. Glucose consumption and lactic acid production were tested, and lactate dehydrogenase (LDHA), pyruvate kinase m2 (PKM2), and miR-21 expression were examined. The level of glycolysis in CAFs was determined after treatment with a miR-21 inhibitor. Primary miR-21-NC CAFs and miR-21-inhibitor CAFs were indirectly co-cultured with BxPc-3 in vitro, and the invasion capacity of these cells was determined. The aerobic oxidation index of cancer cells and the expression of succinodehydrogenase (SDH) and fumarate hydratase (FH) were assessed. Compared with NFs, CAFs showed enhanced glucose uptake capacity, lactic acid production, and elevated LDHA, PKM2, and miR-21 expression. After miR-21 inhibitor treatment, the extent of glycolysis in CAFs was reduced. After indirect co-culture with CAFs, oxidative phosphorylation and SDH, FH, and MCT expression increased in BxPc-3 cells. After co-culture with miR-21-inhibitor-CAFs, oxidative phosphorylation and invasion ability of the pancreatic can...Continue Reading
Citations
Jul 26, 2019·Current Molecular Medicine·Praveen SharmaSandeep Singh
May 30, 2020·Cancers·Laura Santana-VieraCarla L Esposito
Oct 23, 2019·Journal of Ovarian Research·Yingyu DouSongshu Xiao
Aug 8, 2020·Frontiers in Cell and Developmental Biology·Maria-Theodora MelissariVasiliki Koliaraki
Jan 23, 2019·Molecular Cancer·Divya Thomas, Prakash Radhakrishnan
Aug 23, 2019·Cellular and Molecular Life Sciences : CMLS·Jinshou YangYupei Zhao
Aug 14, 2019·Frontiers in Pharmacology·Kai-Ching TayBey-Hing Goh
Mar 4, 2020·Molecular Cancer·Cheng QinYupei Zhao
Jun 12, 2019·Cancer Microenvironment : Official Journal of the International Cancer Microenvironment Society·Mohd RihanAmit Khairnar
Dec 1, 2020·The FEBS Journal·Hussein FarahSimon W Jones
Dec 21, 2020·Pathology, Research and Practice·Sina TaefehshokrBehzad Baradaran
Nov 28, 2020·Cancer Letters·Susi ZhuCong Peng
Jan 7, 2021·Cancers·Federico VirgaFrancesca Orso
Feb 13, 2021·Cancers·Martina Tassinari, Paolo Gandellini
Mar 7, 2021·International Journal of Molecular Sciences·Rebecca RaueBernhard Brüne
May 1, 2021·Journal of Clinical Medicine·Zainab L RaiBrian R Davidson
Jun 1, 2021·Epigenetics : Official Journal of the DNA Methylation Society·I Gede Putu SupadmanabaElisa Giovannetti
Jul 3, 2021·Cancers·Xu WangWeiqi Sheng
Jul 3, 2021·Materials·Cristiana Maria GrapaTeodora Mocan
Oct 1, 2021·Cancer Metastasis Reviews·Lorenzo F SempereThomas D Schmittgen
Jul 6, 2021·Signal Transduction and Targeted Therapy·Shun WangQiong-Zhu Dong
Dec 3, 2021·Cancer Metastasis Reviews·Henriette Berg AndersenDominika Czaplinska