miR-21 modulates resistance of HR-HPV positive cervical cancer cells to radiation through targeting LATS1

Biochemical and Biophysical Research Communications
Shikai LiuFangyuan Gao

Abstract

Although multiple miRNAs are found involved in radioresistance development in HR-HPV positive (+) cervical cancer, only limited studies explored the regulative mechanism of the miRNAs. miR-21 is one of the miRNAs significantly upregulated in HR-HPV (+) cervical cancer is also significantly associated with radioresistance. However, the detailed regulative network of miR-21 in radioresistance is still not clear. In this study, we confirmed that miR-21 overexpression was associated with higher level of radioresistance in HR-HPV (+) cervical cancer patients and thus decided to further explore its role. Findings of this study found miR-21 can negatively affect radiosensitivity of HR-HPV (+) cervical cancer cells and decrease radiation induced G2/M block and increase S phase accumulation. By using dual luciferase assay, we verified a binding site between miR-21 and 3'-UTR of large tumor suppressor kinase 1 (LATS1). Through direct binding, miR-21 can regulate LATS1 expression in cervical cancer cells. LATS1 overexpression can reverse miR-21 induced higher colony formation rate and also reduced miR-21 induced S phase accumulation and G2/M phase block reduction under radiation treatment. These results suggested that miR-21-LATS1 axis pl...Continue Reading

References

Jul 5, 2003·Lancet·Steven E Waggoner
Dec 23, 2006·Nature Reviews. Cancer·Ciaran B J WoodmanLawrence S Young
Feb 4, 2010·Cancer Research·Xiaoxia HuXiaowei Wang
May 28, 2011·Biochimica Et Biophysica Acta·Zhi-Ming Zheng, Xiaohong Wang
Oct 18, 2011·Biochimica Et Biophysica Acta·Tingting Yao, Zhongqiu Lin
May 30, 2012·The Journal of Cell Biology·Tatsuyuki ChiyodaShinji Kuninaka
Nov 29, 2013·Cancer Cell International·Bin ZhangJunye Liu
Jan 9, 2014·CA: a Cancer Journal for Clinicians·Rebecca SiegelAhmedin Jemal

❮ Previous
Next ❯

Citations

Aug 6, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Shikai LiuLiang Zhang
Oct 27, 2016·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Lili SongQian Li
Sep 30, 2016·Molecular Diagnosis & Therapy·Eeva Auvinen
Mar 23, 2017·Disease Markers·Parwez AhmadOndrej Slaby
Jan 11, 2021·Pathology, Research and Practice·Kaveh EbahimzadehSoudeh Ghafouri-Fard
Jan 28, 2021·RNA Biology·Guitian HeYadong Zheng
Aug 31, 2021·Seminars in Radiation Oncology·Michael T SpiottoSteven J Frank

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis