MiR-21 nanocapsules promote early bone repair of osteoporotic fractures by stimulating the osteogenic differentiation of bone marrow mesenchymal stem cells

Journal of Orthopaedic Translation
Xiaolei SunXinlong Ma

Abstract

The healing of osteoporotic fractures in the elderly patients is a difficult clinical problem. Currently, based on the internal fixation of fractures, the available drug treatments mainly focus on either inhibiting osteoclast function, such as bisphosphonate, calcitonin, oestrogen or promoting osteogenesis, such as parathyroid hormones. However, the availability of current antiosteoporotic drugs in promoting osteoporotic fracture healing is limited. The objective of the present study was to investigate the ability of the MiR-21/nanocapsule to enhance the early bone repair of osteoporotic fractures. Based on the presence of matrix metalloproteinases that are overexpressed at the fracture site, we designed the matrix metalloproteinase-sensitive nanocapsules which were formed by in situ free radical polymerisation on the surface of MiR-21 with 2-(methacryloyloxy) ethyl phosphorylcholine and the bisacryloylated VPLGVRTK peptide. The MiR-21/nanocapsule [n (miR-21)] and O-carboxymethyl chitosan (CMCS) were mixed until they formed a gel-like material [CMCS/n (miR-21)] with good fluidity and injectability. Thirty elderly Sprague Dawley (SD) rats (female, 14-month-old, 380 ± 10 g) were subjected to bilateral removal of the ovaries (ovar...Continue Reading

References

Jun 14, 2002·The Journal of Bone and Joint Surgery. American Volume·Jay R LiebermanThomas A Einhorn
Aug 7, 2004·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Vera LuginbuehlBruno Gander
May 25, 2006·The Journal of Bone and Joint Surgery. British Volume·A H R W SimpsonB Noble
Apr 15, 2008·Archives of Biochemistry and Biophysics·Koichi Matsuo, Naoko Irie
Nov 26, 2008·Bulletin of Experimental Biology and Medicine·K B KurakhmaevaR N Alyautdin
Mar 20, 2012·Current Osteoporosis Reports·Natalie A Sims, Nicole C Walsh
Nov 22, 2013·Wiley Interdisciplinary Reviews. RNA·Hongwei LiangXi Chen
Sep 19, 2014·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·Hongqiu LiJing Zhang
Nov 18, 2014·Advanced Materials·Chaoyong LiuChunsheng Kang
Mar 3, 2015·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Yu-Bin MengXian-Jin Yang
Jan 8, 2016·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Jianhou HuangYongfei Guo
Jun 25, 2016·Injury·Wing Hoi CheungVolker Alt
Nov 29, 2017·Osteoporosis International : a Journal Established As Result of Cooperation Between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·Julien Paccou, Bernard Cortet
Dec 2, 2017·Nucleic Acids Research·Tomas TokarIgor Jurisica
Jan 18, 2018·Current Osteoporosis Reports·Hanna Taipaleenmäki
Mar 24, 2018·International Journal of Pharmaceutics·Elisabet Segredo-MoralesCarmen Évora
May 3, 2019·Open Access Macedonian Journal of Medical Sciences·Valentina KoevskaBiljana Kalcovska
May 23, 2019·European Cells & Materials·R M WongW H Cheung
May 28, 2019·Der Unfallchirurg·Eric HesseWolfgang Böcker

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Methods Mentioned

BETA
electrophoresis
dynamic light scattering
transmission electron microscopy
flow cytometry
PCR
X-ray
transmission
confocal microscopy
confocal
Fluorescence

Software Mentioned

ImageJ
SPSS17

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