miR-21 Protects Against Ischemia/Reperfusion-Induced Acute Kidney Injury by Preventing Epithelial Cell Apoptosis and Inhibiting Dendritic Cell Maturation

Frontiers in Physiology
Nana SongXiaoqiang Ding

Abstract

Renal tubular injury and innate immune responses induced by hypoxia contribute to acute kidney injury. Accumulating evidence suggests that miR-21 overexpression protects against kidney ischemia injury. Additionally, miR-21 emerges as a key inhibitor in dendritic cell maturation. Thus, we hypothesized that miR-21 protects the kidney from IR injury by suppressing epithelial cell damage and inflammatory reaction. In this study, we investigated effects of miR-21 and its signaling pathways (PTEN/AKT/mTOR/HIF, PDCD4/NFκ-B) on kidney ischemia/reperfusion (IR) injury in vitro and in vivo. The results revealed that IR increased miR-21, HIF1α, and 2α expression in vivo and in vitro. MiR-21 interacted with HIF1α and 2α through the PTEN/AKT/mTOR pathway. Moreover, inhibition of miR-21 activated PDCD4/NFκ-B pathways, which are critical for dendritic cell maturation. Renal IR triggers local inflammation by inducing the dendritic cell maturation and promoting the secretion of IL-12, IL-6, and TNF-α cytokines. Knockdown of miR-21 intensified the effect of IR on tubular epithelial cell apoptosis and dendritic cell maturation. Our results suggested that IR-inducible miR-21 protects epithelial cells from IR injury via a feedback interaction with ...Continue Reading

References

Dec 4, 2003·Nature Reviews. Immunology·Martien L Kapsenberg
Jun 24, 2004·Nature Reviews. Genetics·Lin He, Gregory J Hannon
Jul 14, 2007·Nature Reviews. Immunology·Adrian E Morelli, Angus W Thomson
Jan 8, 2008·Journal of the American Society of Nephrology : JASN·Peter HillPatrick H Maxwell
Dec 20, 2008·International Immunopharmacology·Vanessa Nunes de PaivaNiels O S Câmara
Oct 31, 2009·Journal of the American Society of Nephrology : JASN·Raghu K Tadagavadi, W Brian Reeves
Apr 21, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Fabio JimenezSeema S Ahuja
Jul 14, 2010·Seminars in Nephrology·Li Li, Mark D Okusa
Jul 24, 2010·Proceedings of the National Academy of Sciences of the United States of America·Jonathan G GodwinJohn Iacomini
Jul 22, 2011·American Journal of Physiology. Renal Physiology·Abolfazl ZarjouGang Liu
Feb 22, 2012·Science Translational Medicine·B Nelson ChauJeremy S Duffield
Aug 3, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Amandeep BajwaMark D Okusa
Oct 25, 2012·The Journal of Clinical Investigation·Li LiMark D Okusa
Mar 8, 2013·American Journal of Physiology. Renal Physiology·Yi FangXiaoqiang Ding
Jun 26, 2013·Nephron. Clinical Practice·Paweena SusantitaphongBertrand L Jaber
Sep 4, 2013·The Journal of Clinical Investigation·Katharina HochheiserChristian Kurts
Nov 12, 2013·Protein & Cell·Ya-Feng LiRongshan Li
Mar 13, 2014·Proceedings of the National Academy of Sciences of the United States of America·Marc ClementGiuseppina Caligiuri
Aug 28, 2014·Physiological Genomics·Xialian XuXiaoqiang Ding
Feb 18, 2015·Frontiers in Immunology·Frederick J Sheedy
Apr 7, 2015·Critical Care Medicine·Ping JiaXiaoqiang Ding
Aug 10, 2015·Toxicology and Applied Pharmacology·Soledad LopezCristobalina Mayorga
Aug 21, 2015·Scientific Reports·Nana SongJerry Yu
Oct 10, 2015·Acta Physiologica·Samuel N HeymanChristian Rosenberger
Nov 1, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·Peter I LoboMark D Okusa
Mar 5, 2016·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Luise GaedeHelge Möllmann
Sep 10, 2016·Mediators of Inflammation·Agnieszka LobodaJozef Dulak
Feb 20, 2017·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Qinqin LinZhenjun Tian
Dec 20, 2017·Scientific Reports·Congcong ChenHuachun Weng

❮ Previous
Next ❯

Citations

Jan 26, 2020·International Journal of Molecular Sciences·Kenji TsujiJun Wada
Jan 31, 2020·Experimental Biology and Medicine·Zhiyu Wang, Wen Zhang
Feb 1, 2020·American Journal of Physiology. Renal Physiology·Yu YanZheng Dong
May 28, 2020·Pharmaceutics·Carl Randall HarrellVladislav Volarevic
Jun 8, 2019·Molecular Medicine Reports·Yang WangZhen Jin
Jan 28, 2020·International Journal of Molecular Medicine·Huizheng MaoMin Wang
Jul 16, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Ping JiaXiaoqiang Ding
Jul 7, 2020·American Journal of Physiology. Renal Physiology·Boxing SuJianxing Li
Dec 14, 2018·Frontiers in Veterinary Science·Osamu IchiiYasuhiro Kon
Nov 30, 2019·Frontiers in Pharmacology·Jose Luis Morgado-PascualMarta Ruiz-Ortega
Aug 9, 2020·International Journal of Molecular Sciences·Chiara Corrado, Simona Fontana
Mar 18, 2020·Frontiers in Immunology·Marco QuagliaVincenzo Cantaluppi
Sep 17, 2020·Stem Cell Reviews and Reports·Xiaoli QianLisha Li
Jun 2, 2020·Frontiers in Pharmacology·Timo Brandenburger, Johan M Lorenzen
Dec 15, 2020·Transplantation Reviews·Jiyue WuWei Wang
Jun 22, 2020·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Soudeh Ghafouri-FardMohammad Taheri
May 18, 2021·Canadian Journal of Kidney Health and Disease·Adrianna DouvrisKevin D Burns
Jul 10, 2021·Clinica Chimica Acta; International Journal of Clinical Chemistry·Ryosuke FujiiKoji Suzuki
Jul 27, 2021·Frontiers in Medicine·Elena Cantero-NavarroMarta Ruiz-Ortega

❮ Previous
Next ❯

Methods Mentioned

BETA
transfection
Assay
light microscopy
protein assay
enzyme-linked immunosorbent assay
flow cytometry
ELISA
PCR

Software Mentioned

Image Measure
FlowJo
Statistical Package for the Social Sciences ( SPSS )

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis