miR-210 Participates in Hepatic Ischemia Reperfusion Injury by Forming a Negative Feedback Loop With SMAD4.

Hepatology : Official Journal of the American Association for the Study of Liver Diseases
Wen-Ming PanJin-Xiang Zhang

Abstract

Hepatic ischemia-reperfusion (IR) injury is a major complication of liver transplantation, resection, and hemorrhagic shock. Hypoxia is a key pathological event associated with IR injury. MicroRNA-210 (miR-210) has been characterized as a micromanager of hypoxia pathway. However, its function and mechanism in hepatic IR injury is unknown. In this study, we found miR-210 was induced in liver tissues from patients subjected to IR-related surgeries. In a murine model of hepatic IR, the level of miR-210 was increased in hepatocytes but not in nonparenchymal cells. miR-210 deficiency remarkably alleviated liver injury, cell inflammatory responses, and cell death in a mouse hepatic IR model. In vitro, inhibition of miR-210 decreased hypoxia/reoxygenation (HR)-induced cell apoptosis of primary hepatocytes and LO2 cells, whereas overexpression of miR-210 increased cells apoptosis during HR. Mechanistically, miR-210 directly suppressed mothers against decapentaplegic homolog 4 (SMAD4) expression under normoxia and hypoxia condition by directly binding to the 3' UTR of SMAD4. The pro-apoptotic effect of miR-210 was alleviated by SMAD4, whereas short hairpin SMAD4 abrogated the anti-apoptotic role of miR-210 inhibition in primary hepatocy...Continue Reading

References

Jun 1, 2002·Science·Liliana Attisano, Jeffrey L Wrana
Mar 3, 2006·Genes & Development·Marco Antonio Valencia-SanchezRoy Parker
May 26, 2006·Nature·Jacques PouysségurNathalie M Mazure
Jan 6, 2009·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·A ShakedUNKNOWN A2ALL Study Group
Apr 4, 2009·The Journal of Biological Chemistry·Ashley J Pratt, Ian J MacRae
Oct 27, 2009·Development·Kristi Wharton, Rik Derynck
Nov 10, 2009·Pharmacology & Therapeutics·Pasquale FasanaroFabio Martelli
May 22, 2010·Journal of the American Society of Nephrology : JASN·Arthur C K ChungHui Y Lan
Sep 8, 2010·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Mahmoud Abu-AmaraAlexander Seifalian
Oct 23, 2010·Molecular Cell·Anthony K L Leung, Phillip A Sharp
Dec 7, 2011·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Qiao YingXianghuo He
Dec 12, 2012·Nature Reviews. Gastroenterology & Hepatology·Yuan ZhaiJerzy W Kupiec-Weglinski
May 30, 2013·Current Vascular Pharmacology·Pasquale FasanaroMaurizio C Capogrossi
Mar 20, 2015·Frontiers in Physiology·Gerhild Euler
Sep 4, 2015·Journal of Hepatology·Junfei HuHongliang Li
Sep 12, 2015·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Ernesto Caballero-GarridoTamara Roitbak
Oct 27, 2015·Pharmacology & Therapeutics·José M Muñoz-FélixJosé M López-Novoa
Mar 29, 2016·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Alan Ka-Lun KaiIrene Oi-Lin Ng
Jul 1, 2017·Bioscience, Biotechnology, and Biochemistry·Hongying DiaoZhuoran Li
Sep 28, 2017·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Mohammed ArifRafeeq P H Ahmed

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Citations

Nov 24, 2020·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Fatemeh Sabet SarvestaniAli-Mohammad Tamaddon
Jun 22, 2020·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Soudeh Ghafouri-FardMohammad Taheri
Apr 12, 2021·International Immunopharmacology·Yun-Hai LuoZhong-Jun Wu

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Methods Mentioned

BETA
immunoprecipitation
PCR
Genome
Flow cytometry
ChIP
confocal microscopy

Software Mentioned

SPSS

Related Concepts

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis