This study examined the underlying mechanism of miR-215-5p in multiple myeloma (MM) at the molecular level. miR-215-5p was upregulated in bone marrow specimens of patients with MM and MM cell lines through real-time polymerase chain reaction, and downregulation of miR-215-5p was related to poor survival of patients with MM. The results of Western blot assay showed that the PI3K/AKT/mTOR signaling pathway was activated in bone marrow specimens of patients with MM. miR-215-5p was found to negatively correlate with runt-related transcription factor 1 (RUNX1) expression in MM clinical bone marrow samples. Therefore, to test the probable mechanisms of the regulation of MM cell proliferation and apoptosis, a miR-215-5p mimics/inhibitors/negative control was transfected into MM cells. The targets of miR-215-5p were estimated using four bioinformatics databases (including miRDB, miRTarBase, Starbase, and Targetscan). Furthermore, enrichment analyses of gene ontology and Kyoto Encyclopedia of Genomes pathway were carried out at the Enrichr website. Cell viability was detected using the MTT method, while apoptosis and cell cycle were measured using flow cytometry. RUNX1, CDK2, CDC25B, cleaved-caspase-3, cleaved-PARP, p-PI3K, PI3K, p-AKT,...Continue Reading
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Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis