MiR-215-5p Reduces Liver Metastasis in an Experimental Model of Colorectal Cancer through Regulation of ECM-Receptor Interactions and Focal Adhesion

Cancers
T MachackovaO Slaby

Abstract

Background: Growing evidence suggests that miR-215-5p is a tumor suppressor in colorectal cancer (CRC); however, its role in metastasis remains unclear. This study evaluates the effects of miR-215 overexpression on the metastatic potential of CRC. Methods: CRC cell lines were stably transfected with miR-215-5p and used for in vitro and in vivo functional analyses. Next-generation sequencing and RT-qPCR were performed to study changes on the mRNA level. Results: Overexpression of miR-215-5p significantly reduced the clonogenic potential, migration, and invasiveness of CRC cells in vitro and tumor weight and volume, and liver metastasis in vivo. Transcriptome analysis revealed mRNAs regulated by miR-215-5p and RT-qPCR confirmed results for seven selected genes. Significantly elevated levels of CTNNBIP1 were also observed in patients' primary tumors and liver metastases compared to adjacent tissues, indicating its direct regulation by miR-215-5p. Gene Ontology and KEGG pathway analysis identified cellular processes and pathways associated with miR-215-5p deregulation. Conclusions: MiR-215-5p suppresses the metastatic potential of CRC cells through the regulation of divergent molecular pathways, including extracellular-matrix-recep...Continue Reading

References

Dec 11, 1999·Nucleic Acids Research·M Kanehisa, S Goto
Jul 1, 2005·Molecular and Cellular Biology·Fabeha FazalPrimal de Lanerolle
Jan 13, 2006·Laboratory Investigation; a Journal of Technical Methods and Pathology·Stephanie A BlairJerrold R Turner
Dec 17, 2008·Cancer Research·Christian J BraunMatthias Dobbelstein
Jul 15, 2011·Clinical Colorectal Cancer·Mihriban KaraayvazJingfang Ju
Mar 30, 2012·Omics : a Journal of Integrative Biology·Guangchuang YuQing-Yu He
Apr 4, 2012·Journal of Cellular and Molecular Medicine·Petra FaltejskovaOndrej Slaby
Oct 30, 2012·Bioinformatics·Alexander DobinThomas R Gingeras
Apr 4, 2014·Bioinformatics·Anthony M BolgerBjoern Usadel
Dec 18, 2014·Genome Biology·Michael I LoveSimon Anders
Mar 17, 2015·Proceedings of the National Academy of Sciences of the United States of America·Matthew F JonesAshish Lal
Oct 3, 2015·Bioinformatics·Konstantin OkonechnikovFernando García-Alcalde
May 31, 2016·World Journal of Gastroenterology : WJG·Jordan E AxelradVijay Yajnik
Aug 4, 2016·Clinical Interventions in Aging·Karen Simon
Feb 22, 2017·World Journal of Hepatology·Hong LuCurtis Klaassen
Mar 31, 2017·Nature·Felipe de Sousa e MeloFrederic J de Sauvage
Aug 16, 2017·World Journal of Gastroenterology : WJG·Iyad A Issa, Malak Noureddine
Jul 25, 2018·Scientific Reports·Antonio AgostiniFrancesca Micci
Sep 20, 2018·International Journal of Molecular Sciences·Lei DingQinghua Cui
Sep 29, 2018·F1000Research·Steven W Wingett, Simon Andrews

❮ Previous
Next ❯

Citations

May 1, 2021·International Journal of Molecular Sciences·Yiwei WangZhenyou Jiang
May 15, 2021·OncoTargets and Therapy·Wubin ZhengWeiwei Tang
Jun 28, 2021·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Yuan ChengXuelei Ma
Jul 21, 2021·Journal of Cardiovascular Pharmacology·Xiaojv XiongJijun Liu

❮ Previous
Next ❯

Methods Mentioned

BETA
transfection
flow cytometry
RNA Assay
Assay
RNA-seq
cDNA library
DNA Assay

Software Mentioned

clusterProfiler
GraphPad
ImageJ
bcl2fastq
Bioconductor
BD FACSuite
TargetScan
R
ZEISS ZEN
DESeq2

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.