MiR-216b is involved in pathogenesis and progression of hepatocellular carcinoma through HBx-miR-216b-IGF2BP2 signaling pathway

Cell Death & Disease
F-y LiuX-p Chen

Abstract

This study aims to investigate the expression status of miRNA-216b in familial hepatocellular carcinoma (HCC) and the correlation between miRNA-216b expression and pathogenesis, as well as the progression of HCC. The expression profile of miRNAs in plasma of peripheral blood between HCC patients with HCC family history and healthy volunteers without HCC family history was determined by microarray. Using real-time quantitative PCR to detect the expression in paired tissues from 150 patients with HCC, miR-216b was selected as its expression value in HCC patients was significantly lower compared with healthy volunteers. Next, miR-216b expression and the clinicopathological features of HCC were evaluated. The effect of miR-216b expression on tumor cells was investigated by regulating miR-216b expression in SMMC-7721 and HepG2 in vitro and in vivo. Finally, we explored mRNA targets of miR-216b. In 150 HCC, 37 (75%) tumors showed reduced miR-216b expression comparing with their adjacent liver tissues. The decreased expression of miR-216b was significantly correlated with tumor volume (P=0.044), HBV infection (P=0.026), HBV DNA quantitative (P=0.001) and vascular invasion (P=0.032). The 5-year disease-free survival and overall rates a...Continue Reading

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Citations

Feb 9, 2016·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Kan Chen, Wenjun Shi
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Methods Mentioned

BETA
PCR
xenograft
transfection
co-immunoprecipitation
flow cytometry
transfections
Assay
immunoprecipitation

Software Mentioned

TargetScan
DIANAmT
ECR browser
SPSS
JASPAR
StepOne Real - Time PCR System
miRWalk
miRanda

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