miR-218 inhibits the migration and invasion of glioma U87 cells through the Slit2-Robo1 pathway

Oncology Letters
Jian-Jun GuShi-Ming Zhang

Abstract

Malignant gliomas are the most common primary brain tumors in adults and are associated with the highest mortality rate. Glioma invasion is one of the most notable causes of the poor prognosis of this cancer. Preventing the invasive behavior of malignant glioma cells by altering effector molecules can significantly improve the prognosis of a patient. microRNAs (miRNAs) are small noncoding RNAs, ~22 nucleotides in length, that are able to function as oncogenes or tumor suppressors in human cancer. In the present study, the expression level of miRNA 218 (miR-218) was found to be markedly downregulated in glioma cell lines and human primary glioma tissues. miR-218 upregulation was found to dramatically reduce the migratory speed and invasive ability of glioma cells. Furthermore, it was demonstrated that ectopic expression of miR-218 in glioma cells resulted in the downregulation of roundabout, axon guidance receptor, homolog 1 (Robo1), upregulation of Slit homolog 2 (Slit2) and the expression of associated proteins following Robo1 knockdown by small interfering RNA. In addition, it was demonstrated that miR-218 inactivated the Slit2-Robo1 pathway through downregulating Robo1 expression by directly targeting the 3'-untranslated reg...Continue Reading

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Citations

Feb 22, 2017·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Peng LiMin Gu
May 13, 2018·The Journal of Immunology : Official Journal of the American Association of Immunologists·Roshini FernandoTerry J Smith
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Jan 9, 2019·Trends in Cancer·Luiz Henrique Medeiros GeraldoFlavia Regina Souza Lima

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Methods Mentioned

BETA
transfection
Assay
protein assay

Software Mentioned

SPSS
TargetScan
ImageJ

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