miR-222 attenuates cisplatin-induced cell death by targeting the PPP2R2A/Akt/mTOR Axis in bladder cancer cells

Journal of Cellular and Molecular Medicine
Li-Ping ZengKun Xia

Abstract

Increased miR-222 levels are associated with a poor prognosis in patients with bladder cancer. However, the role of miR-222 remains unclear. In the present study, we found that miR-222 enhanced the proliferation of both the T24 and the 5637 bladder cancer cell lines. Overexpression of miR-222 attenuated cisplatin-induced cell death in bladder cancer cells. miR-222 activated the Akt/mTOR pathway and inhibited cisplatin-induced autophagy in bladder cancer cells by directly targeting protein phosphatase 2A subunit B (PPP2R2A). Blocking the activation of Akt with LY294002 or mTOR with rapamycin significantly prevented miR-222-induced proliferation and restored the sensitivity of bladder cancer cells to cisplatin. These findings demonstrate that miR-222 modulates the PPP2R2A/Akt/mTOR axis and thus plays a critical role in regulating proliferation and chemotherapeutic drug resistance. Therefore, miR-222 may be a novel therapeutic target for bladder cancer.

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Oct 5, 2016·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Dan-Dan WangJin-Hai Tang
May 19, 2017·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Lei YanKerui Cai
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Methods Mentioned

BETA
PCR
transfection
flow cytometry
Assay
confocal microscopy
lipidation

Software Mentioned

Targetscan
FlowJO
SPSS

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