MiR-26a modulates extracellular matrix homeostasis in cartilage

Matrix Biology : Journal of the International Society for Matrix Biology
Julia EtichBent Brachvogel

Abstract

MicroRNAs (miRNAs) may represent new therapeutic targets for bone and joint diseases. We hypothesized that several cartilage-specific proteins are targeted by a single miRNA and used bioinformatics to identify a miRNA that can modulate extracellular matrix (ECM) homeostasis in cartilage. Bioinformatic analysis of miRNA binding sequences in the 3'-untranslated region (3'-UTR) of target genes was performed to identify a miRNA that could bind to the 3'-UTR of cartilage matrix-related genes. MiRNA expression was studied by quantitative PCR of microdissected growth plate cartilage and binding to the 3'-UTR sequences was analyzed by luciferase interaction studies. Levels of proteins encoded by target genes in cultures of miR-26a mimic- or inhibitor-transfected chondrocytes were determined by FACS or immunoblot analysis. The complementary binding sequence of miR-26a and miR-26b was found in the 3'-UTR of the prehypertrophic/hypertrophic-specific genes Cd200, Col10a1 as well as Col9a1 and Ctgf. Both miRNAs were expressed in cartilage and only miR-26a was downregulated in hypertrophic growth plate cartilage. MiR-26a could interact with the 3'-UTR of Cd200 and Col10a1 in luciferase binding studies, but not with Col9a1 and Ctgf. However, ...Continue Reading

References

Sep 1, 1996·Matrix Biology : Journal of the International Society for Matrix Biology·I GirkontaiteK Von der Mark
Nov 17, 2005·Molecular and Cellular Biology·Bastian BuddeSusanne Grässel
Jan 15, 2008·Matrix Biology : Journal of the International Society for Matrix Biology·Katrin BlumbachFrank Zaucke
Feb 2, 2008·Proceedings of the National Academy of Sciences of the United States of America·Tatsuya KobayashiHenry M Kronenberg
Mar 4, 2008·Matrix Biology : Journal of the International Society for Matrix Biology·Rita DreierSusanne Grässel
Jul 8, 2009·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Daniele BelluoccioBent Brachvogel
Mar 5, 2010·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Daniele BelluoccioBent Brachvogel
Jun 14, 2012·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Ivan GrskovicBent Brachvogel
Nov 20, 2012·Matrix Biology : Journal of the International Society for Matrix Biology·Zina Jeyapalan RutnamBurton B Yang
Nov 30, 2012·The Journal of Investigative Dermatology·Steven N ReulandYiqun G Shellman
Jan 30, 2013·PloS One·Daniel W ThomsonGregory J Goodall
Feb 15, 2013·Arthritis Research & Therapy·Tetsuya MatsukawaNaoki Ishiguro
May 3, 2014·International Journal of Molecular Medicine·De-Sheng JiangHong-Yan Fang

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Citations

Oct 25, 2016·Matrix Biology : Journal of the International Society for Matrix Biology·Evgenia KarousouSpyros S Skandalis
Apr 4, 2018·Yonsei Medical Journal·Chen Xi Yu, Shui Sun
Jun 26, 2020·Development·Qin BianPatrick Cahan
Jun 13, 2020·International Journal of Molecular Sciences·Veronika S GeorgievaBent Brachvogel
Jan 18, 2019·Journal of Cellular Biochemistry·Chunrong ZhaoShiwu Dong
May 16, 2019·The Journal of Cell Biology·Tatjana HolzerBent Brachvogel
Jan 18, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Nader Akbari DilmaghnaiSoudeh Ghafouri-Fard
Apr 15, 2021·Laboratory Investigation; a Journal of Technical Methods and Pathology·Kai ZhouZongke Zhou

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