miR-26a regulates mouse hepatocyte proliferation via directly targeting the 3' untranslated region of CCND2 and CCNE2

Hepatobiliary & Pancreatic Diseases International : HBPD INT
Jian ZhouXiaoshun He

Abstract

The deficiency of liver regeneration needs to be addressed in the fields of liver surgery, split liver transplantation and living donor liver transplantation. Researches of microRNAs would broaden our understandings on the mechanisms of various diseases. Our previous research confirmed that miR-26a regulated liver regeneration in mice; however, the relationship between miR-26a and its target, directly or indirectly, remains unclear. Therefore, the present study further investigated the mechanism of miR-26a in regulating mouse hepatocyte proliferation. An established mouse liver cell line, Nctc-1469, was transfected with Ad5-miR-26a-EGFP, Ad5-anti-miR-26a-EGFP or Ad5-EGFP vector. Cell proliferation was assessed by MTS, cell apoptosis and cell cycle by flow cytometry, and gene expression by Western blotting and quantitative real-time PCR. Dual-luciferase reporter assays were used to test targets of miR-26a. Compared with the Ad5-EGFP group, Ad5-anti-miR-26a-EGFP down-regulated miR-26a and increased proliferation of hepatocytes, with more cells entering the G1 phase of cell cycle (82.70%+/-1.45% vs 75.80%+/-3.92%), and decreased apoptosis (5.50%+/-0.35% vs 6.73%+/-0.42%). CCND2 and CCNE2 were the direct targeted genes of miR-26a. ...Continue Reading

Citations

May 16, 2018·Reproduction in Domestic Animals = Zuchthygiene·M RanB Chen
Aug 1, 2019·International Journal of Molecular Medicine·Jian ZhouXiaoshun He
Jul 21, 2017·Frontiers in Molecular Neuroscience·Shasha ZhangRenjie Chai
Sep 5, 2019·Asian-Australasian Journal of Animal Sciences·Xiaodong ZhangZongjun Yin
Dec 10, 2019·Bioscience, Biotechnology, and Biochemistry·Yingzi ZhangGuanmei Zhu
Dec 12, 2020·British Journal of Pharmacology·Wenqian ZhangDengfeng Gao
Jun 5, 2019·Kidney & Blood Pressure Research·Xiaoyan LiWeiqiang Lin

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