MiR-27a modulates MDR1/P-glycoprotein expression by targeting HIPK2 in human ovarian cancer cells

Gynecologic Oncology
Zhimin LiZehua Wang

Abstract

MicroRNAs (miRNAs) are non-coding, single-stranded small RNAs that regulate gene expression negatively, which is involved in fundamental cellular processes and the initiation, development and progression of human cancer. In this study, we investigated the role of miR-27a in the development of drug resistance in ovarian cancer cells. Expression of miR-27a in ovarian cancer cell lines A2780 and A2780/Taxol were detected by stem-loop real-time PCR. A2780 and A2780/Taxol cells were transfected with the mimics or inhibitors of miR-27a or negative control RNA (NC) by Lipofectamine 2000. The expression levels of MDR1 mRNA, P-glycoprotein (P-gp) and Homeodomain-interacting protein kinase-2 (HIPK2) proteins were assessed by real-time PCR and western blot respectively. Drug sensitivity was analyzed by MTT assay while apoptosis and the fluorescence intensity of intracellular Rhodamine 123 (Rh-123) were measured by FACS. The expression levels of miR-27a and P-gp were up-regulated in paclitaxel-resistant ovarian cancer cell line A2780/Taxol as compared with its parental line A2780. Transfection of A2780/Taxol cells with the inhibitors of miR-27a decreased the expression of MDR1 mRNA and P-gp protein, increased HIPK2 protein expression, enha...Continue Reading

References

Dec 12, 2001·Nature Cell Biology·Thomas G HofmannM Lienhard Schmitz
Jan 10, 2002·Nature Cell Biology·Gabriella D'OraziSilvia Soddu
Oct 8, 2003·The Oncologist·Gregory D LeonardSusan E Bates
Oct 28, 2005·The New England Journal of Medicine·Chang-Zheng Chen
Jan 20, 2006·Nature·Gerhard M SchrattMichael E Greenberg
Oct 31, 2006·Oncogene·M-L SiY-Y Mo
Dec 13, 2006·International Journal of Gynaecology and Obstetrics : the Official Organ of the International Federation of Gynaecology and Obstetrics·A P M HeintzU Beller
Oct 13, 2007·Annals of Oncology : Official Journal of the European Society for Medical Oncology·T Fojo, M Menefee
Sep 27, 2007·Molecular Cancer·William C S Cho
Dec 22, 2007·International Journal of Cancer. Journal International Du Cancer·Elisa BarbarottoGeorge A Calin
Dec 26, 2007·Methods : a Companion to Methods in Enzymology·Christine C Esau
Jan 17, 2008·Nature Reviews. Genetics·Witold FilipowiczNahum Sonenberg
May 2, 2008·International Journal of Cancer. Journal International Du Cancer·Lin XiaDaiming Fan
Jul 23, 2008·Journal of Experimental & Clinical Cancer Research : CR·Rosa PucaGabriella D'Orazi
Jul 23, 2008·Molecular Cancer Therapeutics·Olga KovalchukIgor P Pogribny
Oct 1, 2008·Gynecologic Oncology·Antonio SorrentinoCristiano Ferlini
Oct 7, 2008·Biochemical and Biophysical Research Communications·Yasunori FujitaMasafumi Ito
Jun 9, 2009·Gynecologic Oncology·Xiaoxia HuXiaowei Wang
Jul 28, 2009·International Journal of Cancer. Journal International Du Cancer·Tongsen ZhengLianxin Liu

❮ Previous
Next ❯

Citations

Aug 28, 2012·Epigenomics·Sierk Haenisch, Ingolf Cascorbi
Sep 13, 2012·Pharmacogenomics·Marina KacevskaMagnus Ingelman-Sundberg
Nov 23, 2012·Pharmacogenomics·Adam E FramptonJustin Stebbing
Jun 20, 2014·Future Oncology·Mehmet SevenMustafa Ozen
Apr 30, 2013·Acta Pharmacologica Sinica·Haoran Li, Burton B Yang
Jul 12, 2013·Cancer Biotherapy & Radiopharmaceuticals·Banzhou PanHaizhu Song
Jan 7, 2011·Expert Opinion on Therapeutic Targets·Paolo GandelliniNadia Zaffaroni
Mar 23, 2012·Expert Opinion on Drug Metabolism & Toxicology·Igor KoturbashIgor P Pogribny
Oct 11, 2014·Expert Opinion on Drug Delivery·Khadija RhodaViness Pillay
Jun 27, 2012·Expert Opinion on Biological Therapy·Liqin Du, Alexander Pertsemlidis
Nov 15, 2015·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Ai-Ming YuJoseph L Jilek
Jan 23, 2016·Cancer Biology & Therapy·Katia DanzaStefania Tommasi
Jun 13, 2013·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·Michela Garofalo, Carlo M Croce
Aug 21, 2012·Cancer Treatment Reviews·Arun Kanakkanthara, John H Miller
Dec 6, 2011·Advanced Drug Delivery Reviews·Eleonora AronicaJan A Gorter
Jan 16, 2013·Journal of Oncology·Sreeja SarojiniK Stephen Suh
Mar 14, 2016·Cancer Metastasis Reviews·Jörg HaierRichard Hummel
Oct 11, 2013·Journal of Cellular and Molecular Medicine·Shi-Yun CuiLong-Bang Chen
Dec 3, 2013·Pigment Cell & Melanoma Research·Robert W GeorgantasKoustubh Ranade
Oct 10, 2014·Journal of Ovarian Research·Shuo ChenYang Zhao
Mar 26, 2013·Journal of Ovarian Research·Young Tae ParkHee-Jung An
May 17, 2011·Journal of Experimental & Clinical Cancer Research : CR·Xiaohong ZhaoJianguo Hu
Jun 28, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Dimitry A Chistiakov, Vladimir P Chekhonin
Dec 3, 2014·Advanced Drug Delivery Reviews·Huiyuan WangYongzhuo Huang
Apr 19, 2011·Toxicology Mechanisms and Methods·Curtis D KlaassenJulia Yue Cui
Apr 5, 2016·Cell Biochemistry and Biophysics·Bo JinHaijiang Wang
Apr 24, 2016·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Yogita K Adlakha, Neeru Saini
Jan 30, 2014·Gynecologic Oncology·John K ChanLilly Y W Bourguignon
Apr 10, 2012·European Journal of Pharmacology·Dimitry A Chistiakov, Vladimir P Chekhonin
Sep 28, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Lin LiuZehua Wang
Aug 27, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Can ZongTie-Mei Shi
Sep 1, 2016·Expert Opinion on Drug Metabolism & Toxicology·Takeshi HirotaIchiro Ieiri

❮ Previous
Next ❯

Related Concepts

Related Feeds

Arterial-Venous in Development & Disease

Arterial-venous development may play a crucial role in cardiovascular diseases. Here is the latest research.

BCL-2 Family Proteins

BLC-2 family proteins are a group that share the same homologous BH domain. They play many different roles including pro-survival signals, mitochondria-mediated apoptosis and removal or damaged cells. They are often regulated by phosphorylation, affecting their catalytic activity. Here is the latest research on BCL-2 family proteins.

Antisense Oligonucleotides: ND

This feed focuses on antisense oligonucleotide therapies such as Inotersen, Nusinursen, and Patisiran, in neurodegenerative diseases including amyotrophic lateral sclerosis.

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Cancer Metabolism

In order for cancer cells to maintain rapid, uncontrolled cell proliferation, they must acquire a source of energy. Cancer cells acquire metabolic energy from their surrounding environment and utilize the host cell nutrients to do so. Here is the latest research on cancer metabolism.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.