miR-29c-3p promotes senescence of human mesenchymal stem cells by targeting CNOT6 through p53-p21 and p16-pRB pathways.

Biochimica Et Biophysica Acta
Jin ShangYue Zhou

Abstract

Mesenchymal stem cells (MSCs) are important seed cells for tissue engineering and are promising targets for cell-based therapies. However, the replicative senescence of MSCs during in vitro culture limits their research and clinical applications. The molecular mechanisms underlying the replicative senescence of MSCs are not fully understood. Evidence suggests that miRNAs play important roles in replicative senescence. A microarray analysis found that the miR-29c-3p level was significantly increased during the MSC senescence process. In our study, we investigated the roles of miR-29c-3p in senescence of MSCs. We cultured MSCs for long periods of time, up and down-regulated the miR-29c-3p expression in MSCs, and examined the senescent phenotype changes. The over-expression of miR-29c-3p led to enhanced senescence-associated-β-galactosidase (SA-β-gal) staining, senescence associated secretory phenotype (SASP), senescence associated heterochromatic foci (SAHF), reduced proliferation ability, retarded osteogenic differentiation and corresponding changes in senescence markers, whereas the miR-29c-3p down-regulation had the opposite results. Dual-luciferase reporter assays demonstrated that CNOT6 is the target gene of miR-29c-3p. Knoc...Continue Reading

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Citations

Sep 7, 2019·Current Stem Cell Research & Therapy·Ying Chen, Liling Tang
Jun 2, 2016·American Society of Clinical Oncology Educational Book·Arti HurriaHyman B Muss
Jul 6, 2020·Stem Cell Research & Therapy·Liqiang ZhangWenjia Liu
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Apr 18, 2018·International Journal of Molecular Sciences·Petra MatouškováLenka Skálová
Jun 26, 2020·Frontiers in Cell and Developmental Biology·Xueke ZhouXin Li
Oct 10, 2020·World Journal of Stem Cells·Qing-Shu MengXiao-Ting Liang
Apr 6, 2021·Frontiers in Cell and Developmental Biology·Jingyi CaiZhihe Zhao
May 7, 2021·Stem Cells International·Dina RadyKarim M Fawzy El-Sayed

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