MiR-29c-3p Suppresses the Migration, Invasion and Cell Cycle in Esophageal Carcinoma via CCNA2/p53 Axis

Frontiers in Bioengineering and Biotechnology
Haiyong WangGuangmao Yu

Abstract

In the present study, we tried to describe the role of miR-29c-3p in esophageal carcinoma (EC) and the relationship of miR-29c-3p with CCNA2 as well as cell cycle, accordingly revealing the potential molecular mechanism across cell proliferation, migration and invasion. Expression profiles of EC miRNAs and matched clinical data were accessed from TCGA database for differential and survival analyses. Bioinformatics databases were employed to predict the downstream targets of the potential miRNA, and enrichment analysis was performed on the miRNA and corresponding target gene using GSEA software. qRT-PCR was conducted to detect the expression levels of miR-29c-3p and CCNA2 mRNA in EC tissues and cells, and Western blot was performed for the examination of CCNA2, CDK1 and p53 protein levels. Subsequently, cells were harvested for MTT, Transwell as well as flow cytometry assays to examine cell viability, migration, invasion and cell cycle. Dual-luciferase reporter gene assay and RIP were carried out to further investigate and verify the targeted relationship between miR-29c-3p and CCNA2. MiR-29c-3p was shown to be significantly down-regulated in EC tissues and able to predict poor prognosis. CCNA2 was found to be a downstream targe...Continue Reading

References

Aug 15, 1989·Analytical Biochemistry·P Rao, T N Pattabiraman
Feb 20, 2004·Proceedings of the National Academy of Sciences of the United States of America·George Adrian CalinCarlo M Croce
Jun 10, 2005·Nature·Jun LuTodd R Golub
May 10, 2006·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Farin KamangarWilliam F Anderson
Feb 9, 2012·Journal of Experimental & Clinical Cancer Research : CR·Bao xiang WangNi Yin
May 16, 2012·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Pierre BohanesHeinz-Josef Lenz
Dec 1, 2012·Biomolecular Concepts·Nawal BendrisJean Marie Blanchard
Oct 7, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Danni DengFeng Zhi
Dec 3, 2015·World Journal of Biological Chemistry·Abdelhalim LoukilJean Marie Blanchard
Jan 23, 2016·Journal of Gastroenterology·Kazuto HaradaHideo Baba
Nov 15, 2016·Oncology Reports·Hau-Lun HuangMing-Derg Lai
Nov 14, 2017·Chinese Clinical Oncology·Neeha Zaidi, Ronan J Kelly
Oct 24, 2018·OncoTargets and Therapy·Hai-Ping ZhangJun Lin
Jan 27, 2019·The Journal of Biological Chemistry·Ritu ShekharSandeep Saxena

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Citations

Aug 28, 2020·Frontiers in Pharmacology·Wenfeng ZhangChanggang Sun
Jan 29, 2021·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Xinghong LuoDandan Ma
Dec 18, 2020·Frontiers in Oncology·Soudeh Ghafouri-FardMohammad Taheri

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Datasets Mentioned

BETA
KYSE150
KYSE180

Methods Mentioned

BETA
transfection
electrophoresis
Assay
Protein Immunoprecipitation Assay
RIP
flow cytometry

Software Mentioned

SPSS
GSEA
MSigDB
edgeR

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