MiR-301a-3p Suppresses Estrogen Signaling by Directly Inhibiting ESR1 in ERα Positive Breast Cancer.

Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology
Sandra LettlovaJaroslav Truksa

Abstract

MiRNA-301a-3p is an oncogenic miRNA whose expression is associated with tumor development, metastases and overall poor prognosis. Estrogen receptor α (ERα) is one of the estrogen hormone-activated transcription factors, which regulates a large number of genes and is involved in the mammary gland development. Expression of ERα is considered to be a good indicator for endocrine therapy and breast cancer survival. Loss of ERα in breast cancer patients indicates invasiveness and poor prognosis. In this study, we focus on the regulation of ERα by miR-301a and its role in transition from estrogen-dependent to estrogen-independent breast cancer. Expression of miR-301a-3p was measured by qRT-PCR in tumor tissue samples from 111 patients with primary breast carcinoma and in mammospheres representing in vitro model of cancer stem-like cells. Dual reporter luciferase assay and complementary experiments were performed to validate ESR1 as a direct target of miR-301a-3p. The effect of miR-301a-3p on estrogen signaling was evaluated on the level of gene and protein expression and growth response to estrogens. Finally, the effect of miR-301a-3p expression on tumor growth was studied in nude mice. We identified ESR1 as a direct target of miR-30...Continue Reading

Citations

Mar 21, 2020·Frontiers in Endocrinology·Haojie ZhangYongliang Zhang
Aug 1, 2019·International Journal of Molecular Medicine·Antonio Daniel Martinez-GutierrezAlma D Campos-Parra
Jul 17, 2020·Non-coding RNA·Jun Sheng Wong, Yoke Kqueen Cheah
Feb 21, 2021·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Yingmin WangXingyou Ma
May 25, 2021·Computational Biology and Chemistry·Kevin Nathanael RamantoDavid Agustriawan
Sep 4, 2021·Stem Cells International·Jiahui MaoHuan Yang
Oct 31, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Yuelong ZhangQi Zhang

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