MiR-301a mediates the effect of IL-6 on the AKT/GSK pathway and hepatic glycogenesis by regulating PTEN expression

Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology
Lin DouJian Li

Abstract

IL-6 has been implicated in the pathogenesis of insulin resistance. MiR-301a plays an important role in various biological and pathological processes, including cellular development and differentiation, inflammation, apoptosis and cancer. However, whether miR-301a mediates IL-6-induced insulin resistance in hepatocytes remains unknown. The activation of AKT/GSK pathway and the level of glycogenesis were examed in NCTC 1469 cells transfected miR-301a mimics and inhibitor. Using computational miRNA target prediction database, PTEN was a target of miR-301a. The effect of miR-301a on PTEN expression was evaluated using Luciferase assay and western blot. A PTEN-specific siRNA was used to further determine the effect of PTEN on IL-6-induced insulin resistance. In vivo and in vitro treatment with IL-6 was led to down-regulation of miR-301a, accompanied by impairment of theAKT/GSK pathway and glycogenesis. Importantly, over-expression of miR-301a rescued IL-6-induced decreased activation of the AKT/GSK pathway and hepatic glycogenesis. In contrast, down-regulation of miR-301a induced impaired phosphorylation of AKT and GSK, accompanied by reduced glycogenesis in hepatocytes. Moreover, our results indicate that suppression of PTEN, a ta...Continue Reading

Citations

Mar 22, 2016·The FEBS Journal·Shuyue WangJian Li
May 28, 2016·Molecular Brain·Juan ChenQi Wan
Oct 13, 2016·European Journal of Clinical Nutrition·L DioniV Bollati
Oct 21, 2016·International Journal of Molecular Sciences·Juan FengLan Xie
Dec 20, 2017·Molecular Medicine Reports·Lin DouJian Li
Aug 23, 2018·Molecular Medicine Reports·Shuyue WangJian Li
Oct 16, 2019·Oxidative Medicine and Cellular Longevity·Que WangJian Li
Feb 6, 2016·International Journal of Oncology·Masanori KawanoHiroshi Tsumura
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Jan 11, 2019·Digestive Diseases and Sciences·Shuyue WangHuiying Zhao
Mar 9, 2017·Omics : a Journal of Integrative Biology·Toshiaki NakanoChao-Long Chen

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