miR-301a promotes intestinal mucosal inflammation through induction of IL-17A and TNF-α in IBD

Gut
Chong HeZhanju Liu

Abstract

MicroRNA (miR)-301a is known to be involved in the tumourigenesis and pathogenesis of several autoimmune diseases, but it remains unclear whether miR-301a is associated with the pathogenesis of IBD. miR-301a expression was assessed in peripheral blood mononuclear cells (PBMC) and inflamed mucosa of patients with IBD by quantitative real-time-PCR. Peripheral blood CD4+ T cells were transduced with lentivirus-encoding pre-miR-301a (LV-miR-301a) or a reverse complementary sequence of miR-301a (LV-anti-miR-301a), and their differentiation and activation were investigated in vitro. Antisense miR-301a was administered into mice during trinitrobenzene sulphonic acid (TNBS)-induced colitis to determine its role in colitis. miR-301a expression was significantly upregulated in PBMC and inflamed mucosa of patients with IBD compared with healthy controls. Stimulation with tumour necrosis factor-α (TNF-α) significantly enhanced miR-301a expression in IBD CD4+ T cells, which was markedly reversed by anti-TNF-α mAb (Infliximab) treatment. Transduction of LV-miR-301a into CD4+ T cells from patients with IBD promoted the Th17 cell differentiation and TNF-α production compared with the cells with expression of LV-anti-miR-301a. SNIP1 as a functi...Continue Reading

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Citations

Jun 19, 2019·Journal of Crohn's & Colitis·Julien VerdierGernot Sellge
Jul 19, 2018·Inflammatory Bowel Diseases·Ross John PorterMairi Hall McLean
Aug 6, 2018·Journal of Cellular Physiology·Golamreza JadideslamAlireza Khabbazi
Nov 19, 2019·Frontiers in Genetics·Zhen ZengHu Zhang
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Nov 22, 2016·Scandinavian Journal of Gastroenterology·Guangxi ZhouZhanju Liu
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Feb 9, 2021·Journal of Immunology Research·Junjun ZhaoQiuran Xu
Apr 4, 2021·Nutrients·María Jesús Rodríguez-SojoAlba Rodríguez-Nogales
Oct 31, 2020·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Juanjuan ZhaoLin Xu
May 11, 2021·Frontiers in Immunology·Junxia HuangJi Yang
May 28, 2021·The Kaohsiung Journal of Medical Sciences·Ling-Ling Huo, Zhao-Rui Sun
May 28, 2021·Frontiers in Cell and Developmental Biology·Fabian Vega-TapiaLoreto Cuitino
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Jul 25, 2021·The Journal of Immunology : Official Journal of the American Association of Immunologists·Wenjing YangYingzi Cong

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