MiR-302e attenuates allergic inflammation in vitro model by targeting RelA

Bioscience Reports
Lifeng XiaoYuru Li

Abstract

Allergic inflammation is the foundation of allergic rhinitis and asthma. Although microRNAs are implicated in the pathogenesis of various diseases, information regarding the functional role of microRNAs in allergic diseases is limited. Herein, we reported that microRNA-302e (miR-302e) serves as an important regulator of allergic inflammation in human mast cell line, HMC-1 cells. Our results showed that miR-302e is the dominant member of miR-302 family expressed in HMC-1 cells. Moreover, the expression of miR-302e was significantly decreased in response to phorbol 12-myristate 13-acetate (PMA) and calcium ionophore A23187 or ovalbumin (OVA) stimulation. Overexpression of miR-302e blocked PMA/A23187 or OVA induced the increase in inflammatory cytokines levels, such as IL-1β, IL-6, tumor necrosis factor (TNF)-α and thymic stromal lymphopoietin, while miR-302 inhibition further promoted the release of these cytokines. Mechanistically, we found that miR-302e is a novel miRNA that targets RelA, a gene known to be involved in regulating inflammation, through binding to the 3'-UTR of RelA mRNA. Ectopic miR-302e remarkably suppressed the luciferase activity and expression of RelA, whereas down-regulation of miR-302e increased RelA lucif...Continue Reading

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Citations

May 23, 2019·International Journal of Molecular Medicine·Shaoxia LiJingtao Li
Sep 17, 2019·International Journal of Molecular Medicine·Shangzeng WangLicheng Wei
Apr 1, 2020·F1000Research·Hwan Soo KimToshiaki Kawakami
Aug 14, 2020·Acta Biochimica Et Biophysica Sinica·Menghuan GuoHong Zhang
May 6, 2019·International Journal of Molecular Sciences·Irit SheflerYoseph A Mekori
Jan 14, 2020·The Journal of Allergy and Clinical Immunology·Yong Sun LeeJin Tae Hong
Sep 19, 2020·European Journal of Pharmacology·Yaqiong ZhuMeiqun Wang
Dec 17, 2019·Molecular Therapy. Nucleic Acids·Xiaomin LiuZhongliang Ma

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Methods Mentioned

BETA
PCR
PMA
Assay
Protein Assay
electrophoresis
nuclear translocation
transfection
ELISA

Software Mentioned

SPSS
ImageJ
TargetScan

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