miR-30a attenuates cardiac fibrosis in rats with myocardial infarction by inhibiting CTGF

Experimental and Therapeutic Medicine
Liwen ChenNailiang Tian

Abstract

The mechanism of miR-30a in myocardial fibrosis in rats with myocardial infarction (MI) was investigated. rAAV9-miR-30a was constructed and transfected to heart via injecting into the left ventricular cavity of MI rats. The sham operation group, control group, miR-30a group and miR-30a-NC group were established. Besides, the 3'-UTR of CTGF was inserted into luciferase expression plasmid (pMir-report), then transfected into COS1 cells. miR-30a and control miRNA were, respectively, cotransfected into COS1 cells. The expression of luciferase was detected before and after knockdown of the binding site of miR-30a and the 3'-UTR of CTGF. Four weeks after MI surgery, cardiac function was measured by color Doppler echocardiography, including short axis shortening (FS) and left ventricular ejection fraction (LVEF); the myocardial collagen volume fraction (CVF) was observed by Masson's staining; deposition of collagen I and collagen III were evaluated by immunohistochemical stain; using real-time PCR to detect expression levels of miR-30a and CTGF; the expression of CTGF was observed by western blotting. In MI group, cardiac function was significantly improved, while the expression levels of CVF, collagen I and III, the ratio of type I/I...Continue Reading

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Citations

Mar 15, 2020·Heart Failure Reviews·Bianca Olivia Cojan-MinzatLucia Agoston-Coldea
Feb 28, 2020·Experimental and Therapeutic Medicine·Shaohua XianZhengfu Zhang
Jan 7, 2021·International Journal of Molecular Sciences·Sara PanizoJorge B Cannata-Andía
Feb 13, 2021·Cells·Serena SilvestroEmanuela Mazzon
Jan 9, 2021·American Journal of Physiology. Heart and Circulatory Physiology·Yan ZhangMingjie Pang
Jan 14, 2022·Canadian Journal of Physiology and Pharmacology·Xiao-Xu Yang, Zhen-Yu Zhao

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Methods Mentioned

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transfection

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