miR-30a Regulates the Expression of CAGE and p53 and Regulates the Response to Anti-Cancer Drugs.

Molecules and Cells
Deokbum ParkDooil Jeoung

Abstract

We have previously reported the role of miR-217 in anti-cancer drug-resistance. miRNA array and miRNA hybridization analysis predicted miR-30a-3p as a target of miR-217. miR-30a-3p and miR-217 formed a negative feedback loop and regulated the expression of each other. Ago1 immunoprecipitation and co-localization analysis revealed a possible interaction between miR-30a-3p and miR-217. miR-30a-3p conferred resistance to anti-cancer drugs and enhanced the invasion, migration, angiogenic, tumorigenic, and metastatic potential of cancer cells in CAGE-dependent manner. CAGE increased the expression of miR-30a-3p by binding to the promoter sequences of miR-30a-3p, suggesting a positive feedback loop between CAGE and miR-30a-3p. miR-30a-3p decreased the expression of p53, which showed the binding to the promoter sequences of miR-30a-3p and CAGE in anti-cancer drug-sensitive cancer cells. Luciferase activity assays showed that p53 serves as a target of miR-30a. Thus, the miR-30a-3p-CAGE-p53 feedback loop serves as a target for overcoming resistance to anti-cancer drugs.

References

Apr 2, 2002·Biochemical and Biophysical Research Communications·Bomsoo ChoDoo-Il Jeoung
May 18, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Takashi IwataYutaka Kawakami
Aug 21, 2013·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Brent N RexerCarlos L Arteaga
Jan 23, 2014·Journal of Cellular and Molecular Medicine·Zhifang LiuJihui Jia
Feb 11, 2014·Biochemical and Biophysical Research Communications·Jing FuZhaohui Lv
Mar 19, 2014·PloS One·Luis G Pérez-RivasJosé Lozano
Jul 30, 2014·Molecular and Cellular Endocrinology·Zhe Bao WuWei Guo Zhao
Aug 21, 2014·Journal of Neuro-oncology·Wanghao ChenQiaoyu Li
Oct 7, 2014·Journal of Cellular and Molecular Medicine·Cuiping MaoChangjun Lv

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Methods Mentioned

BETA
immunoprecipitation
transfection
PCR
xenograft
GTPases

Software Mentioned

miRanda
Metamorph
TargetScan
RNAhybrid

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