MiR-30b Attenuates Neuropathic Pain by Regulating Voltage-Gated Sodium Channel Nav1.3 in Rats

Frontiers in Molecular Neuroscience
Songxue SuWeidong Zang

Abstract

Nav1.3 is a tetrodotoxin-sensitive isoform among voltage-gated sodium channels that are closely associated with neuropathic pain. It can be up-regulated following nerve injury, but its biological function remains uncertain. MicroRNAs (miRNAs) are endogenous non-coding RNAs that can regulate post-transcriptional gene expression by binding with their target mRNAs. Using Target Scan software, we discovered that SCN3A is the major target of miR-30b, and we then determined whether miR-30b regulated the expression of Nav1.3 by transfecting miR-30b agomir through the stimulation of TNF-α or by transfecting miR-30b antagomir in primary dorsal root ganglion (DRG) neurons. The spinal nerve ligation (SNL) model was used to determine the contribution of miR-30b to neuropathic pain, to evaluate changes in Nav1.3 mRNA and protein expression, and to understand the sensitivity of rats to mechanical and thermal stimuli. Our results showed that miR-30b agomir transfection down-regulated Nav1.3 mRNA stimulated with TNF-α in primary DRG neurons. Moreover, miR-30b overexpression significantly attenuated neuropathic pain induced by SNL, with decreases in the expression of Nav1.3 mRNA and protein both in DRG neurons and spinal cord. Activation of Nav...Continue Reading

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Citations

Dec 9, 2017·Journal of Cellular Physiology·Hui JinDao-Lin Xia
Jul 8, 2017·Journal of Cellular Biochemistry·Li-Juan JiQiang-Min Huang
Jul 31, 2018·Journal of Cellular Biochemistry·Yongfen BaoShigang Shan
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Aug 25, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Li-Ying ZhanQiang-Min Huang

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Methods Mentioned

BETA
Assay
transfection
PCR
protein assay
electrophoresis

Software Mentioned

Target
Scan
Image Lab
NIH Image J
Target Scan

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