miR-30c Impedes Glioblastoma Cell Proliferation and Migration by Targeting SOX9.

Oncology Research
Shihui LiuSujing Zhuang

Abstract

miR-30c has been acknowledged as a tumor suppressor in various human cancers, such as ovarian cancer, gastric cancer, and prostate cancer. However, the role of miR-30c in glioblastoma (GBM) needs to be investigated. In our study, we found that the expression of miR-30c was significantly downregulated in GBM tissues and cell lines. We found that overexpression of miR-30c inhibited cellular proliferation of GBM cells in vitro and in vivo. More GBM cells were arrested in the G0 phase after miR-30c overexpression. Moreover, we showed that miR-30c overexpression suppressed the migration and invasion of GBM cells. Mechanistically, we found that SOX9 was a direct target of miR-30c in GBM cells. Overexpression of miR-30c inhibited the mRNA and protein levels of SOX9 in GBM cells. Moreover, there was a negative correlation between the expression of miR-30c and SOX9 in GBM tissues. Finally, we showed that restoration of SOX9 in GBM cells reversed the proliferation, migration, and invasion of GBM cells transfected with miR-30c mimic. Collectively, our results demonstrated that miR-30c suppressed the proliferation, migration, and invasion of GBM cells via targeting SOX9.

References

May 23, 2007·Archives of Pathology & Laboratory Medicine·C Ryan Miller, Arie Perry
Oct 24, 2008·The New England Journal of Medicine·Michael A Meyer
May 7, 2010·CA: a Cancer Journal for Clinicians·Erwin G Van MeirJeffrey J Olson
Jun 10, 2011·Current Oncology·J C EasawUNKNOWN Canadian Glioblastoma Recommendations Committee
Mar 17, 2015·Biochemical and Biophysical Research Communications·Koji HiraokaTetsu Akiyama
Apr 9, 2016·Oncoimmunology·David C BinderDerek A Wainwright
Apr 25, 2016·Journal of Neuro-oncology·Andrew W SmithA Gabriella Wernicke
Jul 6, 2017·Molecular Medicine Reports·Ya-Qiang HuangShao-Peng Qiu
Jul 8, 2017·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Jing-Mei CaoKun-Ming Huang
Jul 21, 2017·Cellular & Molecular Biology Letters·Mingxi ZengChangming Kang
Jul 21, 2017·International Braz J Urol : Official Journal of the Brazilian Society of Urology·Zhi-Gang YangYing-Xin Guo
Jul 22, 2017·Molecular Medicine Reports·Jian-Jun QuDe-Zhen Zhou
Jul 25, 2017·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Kun ZhouGang Sun
Jul 25, 2017·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Yiran SiYi Ba
Jul 26, 2017·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Jia-Yong WangYong Dai
Sep 14, 2017·Journal of Cancer Research and Therapeutics·Xia WangDi Wang
Sep 19, 2017·International Journal of Cancer. Journal International Du Cancer·Yang FanXu Zhang

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Citations

Sep 19, 2020·Cancer Control : Journal of the Moffitt Cancer Center·Burt P Kotler, Joel S Brown
May 7, 2021·International Journal of Biological Macromolecules·Milad AshrafizadehMichael R Hamblin
Aug 21, 2020·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Milad AshrafizadehMasoud Najafi

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Methods Mentioned

BETA
surgical resection
Infection
transfection
scraping
reverse transcription PCR
xenograft
flow cytometry

Software Mentioned

SPSS
GraphPad Prism

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