miR-324-3p promotes gastric cancer development by activating Smad4-mediated Wnt/beta-catenin signaling pathway

Journal of Gastroenterology
Guangli SunZekuan Xu

Abstract

Emerging evidence suggested that miRNAs can function as oncogenes or tumor suppressors by regulating downstream target genes. miR-324-3p has been reported to function in several carcinomas, but its role in gastric cancer (GC) is still unknown. This study aims to explore the effects of miR-324-3p on the development of GC. Expression of miR-324-3p was examined in GC cells and tissues by qRT-PCR. Effects of miR-324-3p on GC cells were evaluated by cell vitality assay, colony formation assay, cell migration assay, and flow cytometric assay. The dual luciferase assay was used to verify whether miR-324-3p could interact with the potential target genes. Western blot was used to assess the expression level of Smad4 and beta-catenin. Intracellular ATP level was also examined. The tumor xenografts were established using nude mice. A gastric organoid model was made from fresh stomach tissue. miR-324-3p was expressed at higher levels in the tumor tissues compared with adjacent normal tissues. Overexpression of miR-324-3p promoted cell growth, migration, and decreased apoptosis. miR-324-3p repressed the expression of Smad4, and loss of Smad4 activated the Wnt/beta-catenin signaling pathway. Overexpression of Smad4 rescued the effects of miR...Continue Reading

References

Aug 3, 2002·Science·György Hutvágner, Phillip D Zamore
Jan 28, 2006·World Journal of Gastroenterology : WJG·Ling Yang
Mar 3, 2006·Genes & Development·Marco Antonio Valencia-SanchezRoy Parker
Jan 4, 2007·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Li-Hui WangYoung Kee Shin
Apr 25, 2008·Nature Reviews. Cancer·Alexandra Klaus, Walter Birchmeier
Feb 24, 2009·Critical Reviews in Oncology/hematology·Vincenzo CatalanoEric Van Cutsem
Mar 17, 2009·Biochemical and Biophysical Research Communications·Xiaoxiao TianYali Zhang
Jul 28, 2009·Annual Review of Medicine·Ramiro GarzonCarlo M Croce
Jul 12, 2011·Physiological Reviews·Danish Sayed, Maha Abdellatif
Jun 12, 2012·Cell·Hans Clevers, Roel Nusse
Dec 22, 2012·Nature Reviews. Cancer·Jamie N Anastas, Randall T Moon
Feb 11, 2014·Cancer Letters·Wen ZengYiyong Wei
May 3, 2014·Molecular Cancer Research : MCR·Jun Won ParkHark Kyun Kim
Aug 8, 2014·Anti-cancer Agents in Medicinal Chemistry·Bryan T OronskyJan J Scicinski
Aug 12, 2014·Current Protocols in Mouse Biology·Maxime M MahéNoah F Shroyer
Sep 3, 2014·Journal of Experimental & Clinical Cancer Research : CR·Xiao WangLiping Xie
Sep 16, 2014·International Journal of Cancer. Journal International Du Cancer·Jacques FerlayFreddie Bray
Sep 23, 2014·Gastric Cancer : Official Journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association·Jian-Guo WuRen Zhou
Feb 6, 2015·CA: a Cancer Journal for Clinicians·Lindsey A TorreAhmedin Jemal
Apr 8, 2015·International Journal of Cancer. Journal International Du Cancer·Yang WenZhibin Hu
Sep 20, 2015·Gastroenterology·Manuel Amieva, Richard M Peek
Dec 26, 2015·Experimental Cell Research·Jee-Hye ShinSun-Ho Kee
Jun 18, 2016·Cell·Hans Clevers
Dec 7, 2016·International Journal of Oncology·Jin-Hee LeeYoung Kee Shin
Dec 21, 2016·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Shuang LiYi Ba
Jan 5, 2017·Nature·Kyle W McCrackenJames M Wells
Jan 6, 2017·Cancer Cell International·Chao LiuYuanzheng Qiu
Jan 21, 2017·Science·Annelien L BredenoordJuergen A Knoblich

❮ Previous
Next ❯

Citations

Nov 19, 2019·Journal of Cellular and Molecular Medicine·Jing GanDezhi Mu
Apr 12, 2020·Experimental Biology and Medicine·Yushuang YaoAiping Chen
Jul 28, 2019·Journal of Cellular Physiology·Fatemeh RafieeniaSeyed Hamid Aghaee-Bakhtiari
Jun 4, 2020·Cancer Cell International·Tiancheng ZhaoYezhou Li
Jun 6, 2018·Journal of Experimental & Clinical Cancer Research : CR·Li-Dong ZuGuo-Hui Fu
Nov 5, 2020·Diagnostics·Fatimat KipkeevaAlexander Karpukhin
Nov 18, 2020·Journal of Experimental & Clinical Cancer Research : CR·Guangli SunZekuan Xu
Aug 21, 2019·Molecular Therapy. Nucleic Acids·Yanni GeDongshan Zhang
Feb 2, 2021·Acta Biochimica Et Biophysica Sinica·Yifeng HouHui Lin
Mar 5, 2021·Biochemical and Biophysical Research Communications·Shi-Hua DengYing Xu
Aug 12, 2021·Pediatric Hematology and Oncology·Yishu LuoJun Zhao
Feb 3, 2021·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Chen LuShenglin Ge

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
transfection
flow cytometry
Assay
electrophoresis
xenograft

Software Mentioned

FOPflash
Statistical Product and Service Solutions ( SPSS )
TOPflash
miRanda
CELL Quest
Targetscan

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Related Papers

Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology
Yi ChenJiefu Huang
Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine
Jianxin JiangChengyi Sun
© 2022 Meta ULC. All rights reserved