MiR-324-5p reduces viability and induces apoptosis in gastric cancer cells through modulating TSPAN8

The Journal of Pharmacy and Pharmacology
Hai LinJian-Xiang Gu

Abstract

The purpose of this study was to further clarify the role and underlying mechanism of miR-324-5p in gastric cancer. The expressions of miR-324-5p and TSPAN8 as determined by qRT-PCR or Western blot were compared between the gastric cancer tissues and normal tissues. Human gastric cancer cell line SGC-7901 was cultured and transfected with miR-324-5p mimic/inhibitor or pcDNA-TSPAN8. The cell survival was assessed by the cell viability and apoptosis. Luciferase reporter gene assays were performed to explore the interaction between miR-324-5p and TSPAN8 in SGC-7901 cells. MiR-324-5p was decreased in human gastric carcinoma tissues (n = 33), but TSPAN8 protein expression was increased in the gastric carcinoma tissues (n = 33). Moreover, miR-324-5p inhibited the viability and induced the apoptosis of gastric cancer cells in vitro. TSPAN8 is a functional target of miR-324-5p in gastric cancer. MiR-324-5p was further confirmed to reduce gastric cancer cell viability and induce apoptosis via downregulating TSPAN8 in SGC-7901 cells in vitro. Additionally, miR-324-5p overexpression markedly inhibited the tumorigenesis of gastric cancer cells in vivo, as shown by the smaller tumour volume compared with the control. This study suggested a ...Continue Reading

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Citations

Apr 25, 2019·Journal of Medical Virology·Meghna AgrawalSunit K Singh
Jan 22, 2020·The International Journal of Biological Markers·Feifei LiuJun Wang
Feb 7, 2020·The Journal of Pharmacy and Pharmacology·Vahab Alamdari-PalangiHadi Karami
Dec 21, 2019·Artificial Cells, Nanomedicine, and Biotechnology·Xinrong ZhangDongying Liu
May 1, 2020·Biochimica Et Biophysica Acta. Molecular and Cell Biology of Lipids·Jun GuoJian Li
Jul 6, 2021·Frontiers in Oncology·Yue DengHuiming Peng
Jan 16, 2022·Gastric Cancer : Official Journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association·Cheng ZhangLin Shen

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