MiR-328 suppresses the survival of esophageal cancer cells by targeting PLCE1

Biochemical and Biophysical Research Communications
Na HanPengyuan Zheng

Abstract

Esophageal cancer (EC) is the sixth leading cause of death worldwide. Recent studies have highlighted the vital role of microRNAs (miRNAs) in EC development and diagnosis. In our study, qPCR analysis showed that miRNA-328 was expressed at significantly low levels in EC109 and EC9706 cells. The results also showed that overexpression of miR-328 by lentivirus-mediated gene transfer markedly inhibited cell proliferation and invasion, and enhanced apoptosis; whereas, inhibition of miR-328 significantly promoted cell proliferation and invasion, and suppressed apoptosis in EC109 and EC9706 cells. Dual-luciferase reporter assay confirmed that miR-328 directly targeted phospholipase C epsilon 1 (PLCE1) by binding to target sequences in the 3'-UTR. qPCR and Western blot analysis showed that the PLCE1 was overexpressed in EC109 and EC9706 cells. Additionally, we found that miR-328 overexpression decreased PLCE1 mRNA and protein levels, while miR-328 inhibition enhanced the PLCE1 expression. Further analysis showed that PLCE1 overexpression rescued the inhibitory effect of miR-328 on cell proliferation and invasion, and repressed the promotive effect of miR-328 on cell apoptosis. In conclusion, our results suggest that miR-328 suppresses ...Continue Reading

References

Dec 23, 2006·Seminars in Radiation Oncology·Rebecca S Holmes, Thomas L Vaughan
Feb 26, 2011·International Journal of Cancer. Journal International Du Cancer·Jacques FerlayDonald Maxwell Parkin
Jun 15, 2011·Medical Oncology·Xiaoliang WangZhihai Peng
May 11, 2013·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Yun-Zhao ChenFeng Li
May 18, 2013·International Journal of Molecular Sciences·Paola UliviWainer Zoli
Sep 10, 2013·Seminars in Cancer Biology·Naomi S SakaiRebecca C Fitzgerald
Dec 7, 2013·Neuro-oncology·Sabit DelicMarkus J Riemenschneider
Apr 1, 2014·Biochemical and Biophysical Research Communications·Chengrui FuBaosheng Li
May 29, 2014·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Wen-Qing LiPhilip R Taylor
Jul 17, 2014·Trends in Molecular Medicine·Josie HayesSean Lawler
Aug 8, 2014·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·X R MengQ X Fan
May 8, 2015·Cancer Investigation·Aysegül Ilhan-MutluPeter Birner

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Citations

May 18, 2016·Biochemical and Biophysical Research Communications·Xuan Wang, Ying Xia
Feb 22, 2017·Complementary Medicine Research·Yu-Mei CaiJia-Li Li
Jan 28, 2018·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·S SantasusagnaM Monzo
Mar 13, 2019·Basic & Clinical Pharmacology & Toxicology·Xiangdi YuFangxiang Zhang
Jan 8, 2019·Cancer Biomarkers : Section a of Disease Markers·Mingjing ShenZhongheng Xu
Mar 28, 2021·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·Z M BaiL L Tang
Dec 29, 2021·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Chun-Huei LiaoSuh-Hang H Juo

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