MiR-335 acts as a potential tumor suppressor miRNA via downregulating ROCK1 expression in hepatocellular carcinoma

Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine
Hui LiuXueying Long

Abstract

Increasing evidence has suggested that dysregulation of microRNAs (miRNAs) could contribute to tumor progression. Previous miRNA microarray analysis illustrated that miR-335 is downregulated in various cancers; however, the role of miR-335 on hepatocellular carcinoma (HCC) has not been well elucidated. In this study, we investigated the biological functions and molecular mechanisms of miR-335 in human HCC in vitro, discussing whether it could be a therapeutic biomarker of HCC in the future. Four HCC cell lines and samples from 62 patients with HCC were analyzed for the expression of miR-335 by quantitative RT-PCR. Overexpression of miR-335 was established by transfecting mimics into HepG2 and HuH7 cells. Cell proliferation and cell migration were assessed by cell viability assay and transwell assay. Luciferase reporter assay and Western blot were to verify ROCK1 as a novel target gene of miR-335. We observed that miR-335 was downregulated in human HCC tissues and in all four HCC cell lines. The MTT assay revealed that overexpression of miR-335 subsequently inhibited cell growth. Furthermore, the transwell assay also showed significant cell migration inhibition in miR-335 transfectant. The expression of ROCK1 was decreased evide...Continue Reading

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Citations

Jan 20, 2016·International Journal of Molecular Sciences·Ruirui ZhangMinggang Lei
Feb 20, 2016·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Yong WangJindan An
Mar 13, 2016·Biochemical and Biophysical Research Communications·Shilian ZhangLijie Wang
Aug 21, 2016·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Yue-Yuan LaiBo Xu
Oct 9, 2016·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Long-Ji LuoJin-Hai Tang
Jan 14, 2017·Cancer Biomarkers : Section a of Disease Markers·Jianxia HuZhengju Fu
Feb 21, 2019·Briefings in Functional Genomics·Baohong LiuQi Liu
Jun 15, 2018·Molecular Medicine Reports·Tianpeng JiangQingfan Zeng
Jul 11, 2019·Oncology Letters·Zhe JinYahui Liu
Dec 12, 2018·World Journal of Hepatology·Angélique Gougelet
Mar 23, 2019·International Journal of Molecular Sciences·Reina SasakiMitsuhiko Moriyama
Dec 19, 2019·Cancer Metastasis Reviews·Karina Bezerra SalomãoMaría Sol Brassesco
Dec 12, 2018·Journal of Cellular Physiology·Javid Sadri NahandHossein Keyvani
Oct 13, 2017·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Fang WangFlorin M Selaru

Related Concepts

ROCK1 protein, human
MIRN335 microRNA, human
Cell Motility
Cell Survival
Liver Carcinoma
Malignant Neoplasm of Liver
Tumor Markers
Gene Expression Regulation, Neoplastic
Apoptosis, Intrinsic Pathway
RNA, Small Temporal

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