Abstract
miR-335 and miR-34a are two microRNAs (miRNAs) usually downregulated in gastric cancer (GC). But, their exact regulative roles were not fully elucidated. In this study, we studied the association between miR-335 and/or miR-34a expression and overall survival of GC patients and explored the regulative role of miR-335 and -34a over survivin expression and GC cell growth and invasion. Fifty patients with GC were regularly followed up from 2011 to 2015. miRNA microarray was used to examine the expression trend of miRNAs in eight tumor tissue samples and adjacent normal tissue samples. The possible binding site between miR-335 and survivin messenger RNA (mRNA) was predicted using online database and verified using qRT-PCR, Western blot, and dual luciferase assay. The regulative role of miR-335 and miR-34a over GC cell growth, apoptosis, and invasion was studied using Cell Counting Kit-8 (CCK-8) assay, flow cytometry, and transwell assay, respectively. Among the GC patients, low miR-335 or miR-34a expression is associated with higher clinical stage and lymph node metastasis. Patients with low miR-335 or miR-34a had poor overall survival, while those with combined low miR-335 and miR-34a expression had even poorer overall survival. mi...Continue Reading
References
Jan 28, 2004·Cell·David P Bartel
Nov 2, 2004·Nature Reviews. Cancer·Paul D P PharoahDouglas F Easton
Apr 20, 2006·Cancer Letters·N K SahP S Bisen
May 30, 2006·Molecular Cancer Therapeutics·Seiji Fukuda, Louis M Pelus
Dec 14, 2007·Nature Reviews. Cancer·Dario C Altieri
Jun 10, 2008·Cancer Science·Hirofumi YamamotoMorito Monden
Feb 8, 2011·CA: a Cancer Journal for Clinicians·Ahmedin JemalDavid Forman
Aug 9, 2011·Oncogene·Y XuH Xu
Jan 21, 2012·British Journal of Cancer·H KonishiE Otsuji
May 26, 2012·PloS One·Bhavna KumarPawan Kumar
Dec 25, 2012·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Weiguo CaoYunlin Wu
Jan 22, 2013·Cancer·Long CuiJunming Guo
Jun 12, 2013·PloS One·Andreas KriegWolfram T Knoefel
Sep 27, 2013·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Weiguo CaoYunlin Wu
Oct 30, 2013·The FEBS Journal·Xue-Pu SunXueying Sun
Mar 19, 2014·European Journal of Cell Biology·Zhifang LiuJihui Jia
Mar 22, 2014·PloS One·Jing ChenZhenghao Huo
May 9, 2014·International Journal of Oncology·Hailong LiYongning Zhou
May 20, 2014·Bioscience Reports·Yang PengXiao-Ling Wu
Feb 13, 2015·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·Dianzhong GengHonghua Yin
Apr 1, 2015·Biochemical and Biophysical Research Communications·Mingjun ZhangYongchun Cui
Citations
Jun 21, 2016·International Journal of Molecular Sciences·Ming-Ming TsaiKwang-Huei Lin
Jul 2, 2016·Biochemical and Biophysical Research Communications·Xiunan LiXin Tang
Oct 9, 2016·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Long-Ji LuoJin-Hai Tang
Dec 3, 2016·BMC Infectious Diseases·Su HanJianping Cao
Oct 28, 2017·Clinical Epigenetics·Alejandra Sandoval-BórquezAlejandro H Corvalán
Jan 18, 2019·Biological Chemistry·Xingping DaiXia Xu
Feb 23, 2020·IUBMB Life·Alireza Ahadi
Sep 15, 2017·Oncotarget·Yue ZhangYue-Hua Jiang
Oct 17, 2017·Oncotarget·Yuqian LiLihong Li
Feb 13, 2018·Oncology Letters·Yi-Chen WuZhi-Qiang Ling
Mar 6, 2017·Oncology Reports·Haipeng WangXijia Zhu
May 14, 2019·International Journal of Oncology·Peng ZhangBinxian Wang
May 2, 2019·World Journal of Gastrointestinal Oncology·Ana Flávia Teixeira RossiAna Elizabete Silva
Mar 19, 2020·OncoTargets and Therapy·Yingping ZhuJun Jiang
Oct 23, 2019·International Journal of Molecular Sciences·Jennie OngJoost Kluiver
Jan 16, 2021·BMC Cancer·Yan-Ling ChenLing Li
Feb 4, 2021·Biomedicines·Anna TamaniniRoberto Gambari
May 29, 2021·OncoTargets and Therapy·Lingling YeQuan An Zhang