miR-33a-5p modulates TNF-α-inhibited osteogenic differentiation by targeting SATB2 expression in hBMSCs

FEBS Letters
Wenxiang MiHui Huang

Abstract

miRNAs play a number of roles in bone, including mediating the pathological effects of inflammation. Here, we found that miR-33a-5p expression was significantly increased after TNF-α treatment during BMP-2-induced osteogenic differentiation of hBMSCs. Luciferase reporter assays and western blotting demonstrated that special AT-rich sequence-binding protein 2 (SATB2) is a target of miR-33a-5p. Moreover, we show that BMP-2 induces SATB2 expression by interacting with SATB2 directly via the BMP-2-RUNX2 pathway. However, TNF-α first decreases SATB2 expression by inhibiting miR-33a-5p degradation. We thus conclude that miR-33a-5p plays a central role in this complex regulatory network. These findings will help to understand the regulatory role of miR-33a-5p in the inflammatory process.

References

Jan 1, 1969·Scandinavian Journal of Plastic and Reconstructive Surgery·P I BrånemarkA Ohlsson
Jun 6, 2006·Cell·Debra L Ellies, Robb Krumlauf
Jun 14, 2008·Cardiovascular Research·Carmen UrbichStefanie Dimmeler
Sep 9, 2008·Journal of Clinical Periodontology·Lisa J A Heitz-Mayfield
Mar 24, 2009·Clinical Oral Implants Research·Poliana Mendes DuarteFrancisco Humberto Nociti Júnior
Aug 28, 2010·The Spine Journal : Official Journal of the North American Spine Society·Ryan D MuchowPaul A Anderson
Oct 29, 2010·Proceedings of the National Academy of Sciences of the United States of America·Mohammad Q HassanJane B Lian
Jun 4, 2011·Arthritis Research & Therapy·Kristina Kapinas, Anne M Delany
Feb 9, 2012·Journal of Materials Science. Materials in Medicine·Ling XuFuqiang Zhang
May 9, 2012·The Journal of Cell Biology·Jianwen WeiGerard Karsenty
Jan 29, 2014·BMC Molecular Biology·Marlinda HupkesKoen J Dechering
May 23, 2015·Experimental & Molecular Medicine·Surajit PathakIgnazio Castagliuolo
May 29, 2015·Journal of Orthopaedic Surgery and Research·Yuzhong GaoGuangyu Fan
Jun 13, 2015·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·José Luiz Marques-RochaAmelia Marti

❮ Previous
Next ❯

Citations

Jul 16, 2016·Stem Cells and Development·Claudine SeeligerMartijn van Griensven
Jan 11, 2017·Developmental and Comparative Immunology·Xin LiaoJunyuan Chen
Jun 22, 2017·Current Osteoporosis Reports·Steven R Sera, Nicole I Zur Nieden
Aug 29, 2018·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Xiaolin WuHui Huang
May 1, 2021·Biomedicines·Maria P YavropoulouDimitrios Chatzidimitriou

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.