MiR-3606-3p inhibits systemic sclerosis through targeting TGF-β type II receptor

Cell Cycle
Xiangguang ShiJiucun Wang

Abstract

Systemic sclerosis (SSc) is a multisystemic fibrotic disease characterized by excessive collagen deposition and extracellular matrix synthesis. Though transforming growth factor-β (TGF-β) plays a fundamental role in the pathogenesis of SSc, the mechanism by which TGF-β signaling acts in SSc remains largely unclear. Here, we showed that TGF-β type II receptor (TGFBR2) was significantly upregulated in both human SSc dermal tissues and primary fibroblasts. In fibroblasts, siRNA-induced knockdown of TGFBR2 resulted in a reduction of p-SMAD2/3 levels and reduced production of type I collagen. Additionally, functional experiments revealed that downregulation of TGFBR2 yielded an anti-growth effect on fibroblasts through inhibiting cell cycle progression. Further studies showed that miR-3606-3p could directly target the 3'-UTR of TGFBR2 and significantly decrease the levels of both TGFBR2 mRNA and protein. Furthermore, SSc dermal tissues and primary fibroblasts contain significantly reduced amounts of miR-3606-3p, and the overexpression of miR-3606-3p in fibroblasts replicates the phenotype of TGFBR2 downregulation. Collectively, our findings demonstrated that increased TGFBR2 could be responsible for the hyperactive TGF-β signaling o...Continue Reading

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Citations

Sep 11, 2019·Experimental Dermatology·Xiangguang ShiWenyu Wu
Jan 28, 2022·Clinical and Translational Medicine·Xiangguang ShiJiucun Wang

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Methods Mentioned

BETA
biopsy
electrophoresis
transfections
Assay
Protein Assay
flow cytometry
transfection

Software Mentioned

Image J
BD Cellquest
TargetScan

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