miR-361-5p inhibits hepatocellular carcinoma cell proliferation and invasion by targeting VEGFA

Biochemical and Biophysical Research Communications
Wenxian CuiZhirong Kang

Abstract

MicroRNAs (miRNAs) play important roles in the pathogenesis of many types of cancers by negatively regulating gene expression at posttranscriptional level. Here, we found that miR-361-5p is down-regulated in 135 patients with HCV-related hepatocellular carcinoma (HCC). Moreover, the expressions of miR-361-5p were highly correlated with VEGFA in these HCC patients. Further, CCK-8 proliferation assay indicated that miR-361-5p mimics inhibited the cell proliferation of HepG2 and SNU-398 HCC cells. Transwell assay showed that miR-361-5p mimics inhibited the invasion and migration of HepG2 and SNU-398 HCC cells. Luciferase assays revealed that miR-361-5p directly bound to the 3'untranslated region of VEGFA, and western blotting showed that miR-361-5p inhibited the expression of VEGFA. Generally, this study indicated that miR-361-5p is down-regulated in HCC and inhibits proliferation and invasion of HCC cell lines via VEGFA. In future, miR-361-5p will be a potential therapeutic agent for HCC.

References

Feb 20, 2003·RNA·Victor AmbrosThomas Tuschl
Jan 1, 2009·Journal of Global Infectious Diseases·Luis Jesuino de Oliveria AndradeRaymundo Paraná
Jun 26, 2013·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Jinglei MiaoChaoyue Zhang
Feb 5, 2014·Biochemical and Biophysical Research Communications·Dachuang LiuMing Chen
Feb 24, 2015·Biochemical and Biophysical Research Communications·Guanxiong YeDebiao Pan
Mar 20, 2015·Journal of Carcinogenesis·Yezaz Ahmed GhouriBoris Blechacz
Mar 26, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Kinjal R PatelPrabhudas S Patel
Jul 28, 2015·Integrative Biology : Quantitative Biosciences From Nano to Macro·Raghuram DhumpaMichael G Roper
Oct 18, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Kejia WangPing Zhu

Citations

Nov 15, 2017·Journal of Experimental & Clinical Cancer Research : CR·Fei MaBaoliang Guo
Jan 11, 2019·International Journal of Cancer. Journal International Du Cancer·Yehui DuPenghong Song
Dec 19, 2019·Cancer Metastasis Reviews·Karina Bezerra SalomãoMaría Sol Brassesco
Jan 1, 2021·BioMed Research International·Liang-Chun YinLi-Xin Liu

Related Concepts

VEGF165 protein, human
MIRN361 microRNA, human
Cell Motility
Liver Carcinoma
Malignant Neoplasm of Liver
Neoplasm Invasiveness
Poly(A) Tail
RNA, Neoplasm
Receptor Down-Regulation
Gene Targeting

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Cell Migration in Cancer and Metastasis

Migration of cancer cells into surrounding tissue and the vasculature is an initial step in tumor metastasis. Discover the latest research on cell migration in cancer and metastasis here.

Arterial-Venous in Development & Disease

Arterial-venous development may play a crucial role in cardiovascular diseases. Here is the latest research.