MiR-362 suppresses cervical cancer progression via directly targeting BAP31 and activating TGFβ/Smad pathway.

Cancer Medicine
Shuya YangKun Yang

Abstract

BAP31 (B-cell receptor-associated protein 31) is an important regulator of intracellular signal transduction and highly expressed in several cancer tissues or testicular tissues. Our previous study had revealed that elevated BAP31 plays a crucial role in the progress and metastasis of cervical cancer. Even so, the precise mechanism of abnormal BAP31 elevation in cervical cancer has not been fully elucidated. We revealed that the expression of BAP31 was mainly regulated by microRNA-362 (miR-362), which was markedly downregulated in cervical cancer tissues and negatively correlated with clinical tumor staging. Overexpression of miR-362 inhibited cervical cancer cell proliferation and increased the proportion of apoptotic cells. Furthermore, miR-362 reduced the tumor sizes and prolonged mice survival time in xenograft nude mice model. Finally, we demonstrated that the BAP31/SPTBN1 complex regulated tumor progression through the Smad 2/3 pathway under the control of miR-362. Collectively, our findings demonstrated that miR-362 could work as an anti-oncomiR that inhibits proliferation and promotes apoptosis in cervical cancer cells via BAP31 and TGFβ/Smad pathway. Overexpression of miR-362 might be a potential therapeutic strategy f...Continue Reading

References

Apr 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·R J RotelloL E Gerschenson
Oct 20, 2010·The Journal of Pathology·Erik Meulmeester, Peter Ten Dijke
Apr 27, 2011·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Hye Jung BaekLopa Mishra
Jul 15, 2011·Gene Therapy·K A Lennox, M A Behlke
Dec 6, 2011·Breast Cancer : the Journal of the Japanese Breast Cancer Society·Takeshi ImamuraYasumichi Inoue
Jun 12, 2012·Proceedings of the National Academy of Sciences of the United States of America·Imran A BabarFrank J Slack
Jan 3, 2013·International Journal of Cancer. Journal International Du Cancer·Lise Lotte ChristensenClaus L Andersen
Jul 11, 2013·Nature Biotechnology·Aaron Bouchie
Feb 11, 2014·Journal of the National Cancer Institute·Daniel R PrincipePaul J Grippo
Feb 6, 2015·Digestive Diseases and Sciences·Qing-Hui ZhangLing Xu
Apr 24, 2015·Journal of Experimental & Clinical Cancer Research : CR·Shaojuan YuKun Yang
Dec 10, 2015·Molecular Medicine Reports·Xiaowen ZouAifa Tang
Mar 25, 2016·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Haiyan ZhuXueqiong Zhu
May 4, 2017·Cancer·William SmallDavid K Gaffney
May 9, 2018·Journal of Cellular Biochemistry·Chundi GaoChanggang Sun
Jun 28, 2018·BMC Cancer·Barbara PardiniAlessio Naccarati
May 10, 2019·Molecular Therapy. Nucleic Acids·Rui ChenLin Xu
May 15, 2019·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Lili SongQian Li
Oct 30, 2019·Cancer Genomics & Proteomics·Abdullah A AssiriBrian R Overholser

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Citations

May 1, 2021·Biochimie·Esben M Quistgaard

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Methods Mentioned

BETA
transfection
Assay
PCR
flow cytometry
flow
xenograft
xenografts
chip
immunoprecipitation

Software Mentioned

GraphPad Prism
Novoexpress
Genechem
GraphPad
TargetScan
IPA
Compass
R
ProteinSimple
R language

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