miR-367 promotes proliferation and invasion of hepatocellular carcinoma cells by negatively regulating PTEN

Biochemical and Biophysical Research Communications
Xiangrui MengQingxia Fan

Abstract

MicroRNAs play important roles in the carcinogenesis of many types of cancers by inhibiting gene expression at posttranscriptional level. However, the roles of microRNAs in hepatocellular carcinoma, are still unclear. Here, we identified that miR-367 promotes hepatocellular carcinoma (HCC) cell proliferation by negatively regulates its target gene PTEN. The expression of miR-367 and PTEN are significantly inverse correlated in 35 HCC patients. In HCC cell line, CCK-8 proliferation assay indicated that the cell proliferation was promoted by miR-367, while miR-367 inhibitor significantly inhibited the cell proliferation. Transwell assay showed that miR-367 mimics significantly promoted the migration and invasion of HCC cells, whereas miR-367 inhibitors significantly reduced cell migration and invasion. Luciferase assays confirmed that miR-367 directly bound to the 3'untranslated region of PTEN, and western blotting showed that miR-367 suppressed the expression of PTEN at the protein levels. This study indicated that miR-367 negatively regulates PTEN and promotes proliferation and invasion of HCC cells. Thus, miR-367 may represent a potential therapeutic target for HCC intervention.

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Citations

May 16, 2018·Cancer Biomarkers : Section a of Disease Markers·Wei DingXinghua Pan
Aug 14, 2019·Molecular Oncology·Carolini KaidOswaldo Keith Okamoto
Jun 29, 2017·Oncology Reports·Guodong XiaoSida Qin
Aug 14, 2020·Acta Biochimica Et Biophysica Sinica·Menghuan GuoHong Zhang
Sep 5, 2020·Gut·Johann von FeldenAugusto Villanueva
Jul 29, 2020·Cells·Jovana MarkovicAsha Balakrishnan
Aug 25, 2017·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Wei CaiZhen Su

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