miR-370 regulates ISG15 expression and influences IFN-α sensitivity in hepatocellular carcinoma cells

Cancer Biomarkers : Section a of Disease Markers
Zhuo LiuMan Xia

Abstract

Interferon-α (IFN-α) is an adjuvant to chemotherapy and radiotherapy for hepatocellular carcinoma (HCC), but some HCC patients do not respond to treatment with IFN-α. We performed loss-of-function and gain-of-function experiments to examine the role of ISG15 in the IFN-α sensitivity of LH86, HLCZ01, SMMC7721, and Huh7 cell lines and tumor samples. The overexpression of ISG15 reduced apoptosis in Huh7 and LH86 cells in the presence of IFN-α, whereas the shRNA-mediated knock down of ISG15 expression increased apoptosis in both Huh7 and LH86 cells. We identified a putative miR-370 target site in the 3'-UTR in the ISG15 mRNA, and the level of miR-370 expression in HCC cell lines reflected the level of IFN-α-induced apoptosis exhibited by each. Both HCC cell lines and tumor samples had significantly lower levels of miR-370 than the control cells and tissues (P< 0.05). The overexpression of miR-370 in IFN-α-treated LH86 and Huh7 cells increased apoptosis and reduced the volume of LH86- and Huh7-derived xenograft tumors in mice treated with IFN-α compared with the control tumors. Our findings suggest that miR-370 functions as an HCC tumor suppressor and regulator of IFN-α sensitivity and that miR-370 might be a useful prognostic marke...Continue Reading

References

Apr 30, 2003·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Haizhen ZhuChen Liu
Jul 10, 2003·Journal of Gastrointestinal Surgery : Official Journal of the Society for Surgery of the Alimentary Tract·Lu WangZhao-You Tang
Sep 6, 2005·Biochemical and Biophysical Research Communications·Nadia V GiannakopoulosDong-Er Zhang
Apr 28, 2006·British Journal of Cancer·J B AndersenT F Orntoft
Nov 30, 2006·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Vincenzo MazzaferroUNKNOWN HCC Italian Task Force
Oct 19, 2007·Nature·Irene M PedersenMichael David
Aug 3, 2011·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Alexandru V OlaruFlorin M Selaru
Nov 8, 2011·Cancer Immunology, Immunotherapy : CII·Laurence M WoodYvonne Paterson
Oct 31, 2012·International Journal of Molecular Sciences·Umar-Faruq CajeeMonde Ntwasa
Dec 6, 2012·Clinical Pharmacology and Therapeutics·S P Nana-Sinkam, C M Croce
May 1, 2013·Urologic Oncology·Donald LammJ Alfred Witjes
Jun 1, 2013·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Conghai FanLei Zhang
Jun 4, 2013·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Wen-Ping XuWei-Fen Xie
Jul 9, 2013·Trends in Molecular Medicine·Marco DuranteKathryn D Held
Sep 24, 2013·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Wu YungangXinliang Pan
Oct 26, 2013·Liver Cancer·Alexander G MiamenLewis R Roberts
Mar 26, 2014·International Journal of Radiation Oncology, Biology, Physics·Christopher A Barker, Michael A Postow
Nov 5, 2014·Cancer Research·Bruno SainzSusana Guerra
Sep 25, 2016·Journal of Medical Virology·Hongxia FanHua Tang

❮ Previous
Next ❯

Methods Mentioned

BETA
transfection
xenograft
Assay
PCR
fluorescence
flow cytometry
fluorescence microscopy

Software Mentioned

PicTar
Cell Quest
SPSS

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Carcinoma, Hepatocellular

Hepatocellular Carcinoma is a malignant cancer in liver epithelial cells. Discover the latest research on Hepatocellular Carcinoma here.