miR-372 suppresses tumour proliferation and invasion by targeting IGF2BP1 in renal cell carcinoma

Cell Proliferation
Xuan HuangYong Li

Abstract

MicroRNAs (miRNAs) are endogenous small non-coding RNAs that regulate proteins and mRNAs for degradation or translational suppression. Up to now, the role of miR-372 in renal cell carcinoma has remained unknown; in this study, we have aimed to reveal its functional importance in this tumour. qRT-PCR was performed to measure expression levels of miR-372 in renal cell carcinoma cell lines and tissues. CCK-8 and an invasion assay were performed to measure its functional role. Luciferase assays, qRT-PCR and western blotting were performed to discover miR-372's target gene. We demonstrated that miRNA-372 was down-regulated in renal cell carcinoma cell lines and tissue specimens; its over-expression inhibited cell proliferation and invasion. Moreover, we showed that miRNA-372 repressed insulin-like growth factor 2 mRNA-binding protein 1 (IGF2BP1) expression by directly interacting with its putative binding site at the 3'-UTR. Furthermore, ectopic expression of IGF2BP1 significantly reversed suppression of cell proliferation and invasion caused by miR-372 over-expression. Our data indicated that miR-372 seemed to function as a tumour suppressor in renal cell carcinoma progression by inhibiting the IGF2BP1 expression.

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Citations

May 5, 2018·Endocrine-related Cancer·Chiara VerdelliSabrina Corbetta
Jan 27, 2018·Environmental Science and Pollution Research International·Chunhua YanJianmin Huo
Nov 6, 2018·Journal of Cellular Biochemistry·Lihua YanHongmei Guan
Aug 20, 2016·Oncotarget·Jian LiHuaizhi Wang
Apr 4, 2017·Database : the Journal of Biological Databases and Curation·Sankha Subhra DasNishant Chakravorty
Dec 15, 2018·Journal of Cellular Biochemistry·Wanwei JiangPengbo Zhang
Jul 8, 2020·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Ge SongXueqiang Wang
Dec 21, 2018·Journal of Cellular and Molecular Medicine·Lisheng XieMin Ni
Jan 1, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Yongping ZhouXiaoming Ai
Dec 17, 2020·International Journal of Molecular Sciences·Shu-Chun LinKuo-Wei Chang
Dec 18, 2020·Frontiers in Oncology·Soudeh Ghafouri-FardMohammad Taheri
May 18, 2020·Anti-cancer Agents in Medicinal Chemistry·Marwa H SolimanAbdel-Hady A Abdel-Wahab

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