miR-374a Regulates Inflammatory Response in Diabetic Nephropathy by Targeting MCP-1 Expression

Frontiers in Pharmacology
Zijun YangLin Tang

Abstract

The microRNA (mir)-374a has been implicated in several types of human cancer; however, its role in diabetic nephropathy (DN) remains unclear. Monocyte chemoattractant protein (MCP)-1 is a chemokine that recruits macrophages to inflammatory sites and is important for the development and progression of DN. However, the relationship between miR-374a and MCP-1 in DN is unknown. We addressed this in the present study by examining the expression of these factors in kidney tissue samples from DN patients and through loss- and gain-of-function experiments using HK2 human renal tubular epithelial cells. We found that miR-374a was downregulated whereas MCP-1 was upregulated in DN tissue. A bioinformatics analysis revealed that MCP-1 is a putative target of miR-374a. To confirm this relationship, HK2 cells treated with normal glucose (5.6 mmol/l D-glucose), high glucose (HG) (30 mmol/l D-glucose), or high osmotic pressure solution (5.6 mmol/l D-glucose + 24.4 mmol/l D-mannitol) were transfected with miR-374a mimic or inhibitor. miR-374a mimic reduced MCP-1 mRNA expression and migration of co-cultured U937 cells, whereas miR-374a inhibition had the opposite effects. Additionally, interleukin-6 and -18 and tumor necrosis factor-α levels wer...Continue Reading

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Citations

Mar 21, 2020·Frontiers in Pharmacology·Tamil Selvi LoganathanNoraidatulakma Abdullah
Sep 11, 2020·International Journal of Molecular Sciences·Linsey J F PetersEmiel P C van der Vorst
May 6, 2020·International Journal of Molecular Sciences·Ting-Ting Chang, Jaw-Wen Chen
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Oct 21, 2020·Immunity, Inflammation and Disease·Dexi ZhouJiajie Luan
Feb 6, 2021·International Journal of Molecular Sciences·Sebastian Dommel, Matthias Blüher
Feb 13, 2021·Frontiers in Pharmacology·Yue-Yu GuHui-Yao Lan
Feb 12, 2021·Frontiers in Cell and Developmental Biology·Hong ZhouLi-Qin Tang
Jun 10, 2021·Acta Pharmacologica Sinica·Wen-Xia YangLi Xiao
Jun 20, 2021·Clinical and Experimental Nephrology·Ningmin ZhaoPeizhi Ma
Jul 12, 2021·Arthritis Research & Therapy·Jana BohatáBlanka Stibůrková
Nov 4, 2020·Current Pharmaceutical Design·Stavroula A PaschouDimitrios Tousoulis

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Methods Mentioned

BETA
biopsies
surgical resection
biopsy
Assay
ELISA

Software Mentioned

Microrna
PicTar
SPSS
TargetScan

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