miR-375 Affects the Proliferation, Invasion, and Apoptosis of HPV16-Positive Human Cervical Cancer Cells by Targeting IGF-1R

International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society
Xiao YuMin Hao

Abstract

The aim of this study was to examine the relationship between miR-375 expression and the proliferation, apoptosis, and migration of cervical cancer cells. To further explore the potential target gene of miR-375, insulin-like growth factor 1 receptor (IGF-1R) was detected in miR-375 overexpressed and inhibited cervical cancer cells, which clarified the potential mechanism of miR-375 in the growth and development of cervical cancer. In a cervical cancer cell line (Caski), miR-375 overexpression and knockdown were achieved by transfection with a synthetic miR-375 mimic or miR-375-targeting inhibitor oligonucleotides, respectively, using siRNA-Mate transfection reagents. Real-time Polymerase Chain Reaction was performed to detect the expression level of miR-375. The functional effects of miR-375 on cell proliferation, migration, and apoptosis were evaluated using a Cell Counting Kit (CCK-8) and through scratch wound tests and apoptosis assays, respectively. Western blotting was performed to detect the expression level of the IGF-1R protein. Transfection with the miR-375 mimic significantly upregulated the expression of miR-375 by approximately 7.76-fold (P < 0.05), reduced cell proliferation and migration (P < 0.05), increased apop...Continue Reading

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Sep 27, 2018·Journal of Experimental & Clinical Cancer Research : CR·Huan YanZhan Zhang
Feb 14, 2020·International Journal of Environmental Research and Public Health·Xingyuan WangHongchen Liu
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