MiR-410 Acts as a Tumor Suppressor in Estrogen Receptor-Positive Breast Cancer Cells by Directly Targeting ERLIN2 via the ERS Pathway

Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology
Hewen WuGuohui Wang

Abstract

Endoplasmic reticulum lipid raft-associated 2 (ERLIN2) is reported to be overexpressed in human breast cancer cells and plays an important role in cell proliferation. MicroRNAs (miRNAs) act as post-transcriptional regulators of gene expression and are involved in the development of multiple malignancies, including breast cancer. However, the molecular mechanism of the aberrant ERLIN2 expression in human breast cancer remains poorly understood. MiR-410 expression level was analyzed using Real-time PCR, and ERLIN2 expression was analyzed using Western blot, Real-time PCR and immunohistochemical staining. The effect of miR-410 on ERLIN2 3'UTR intensity was performed using a luciferase assay. Cell proliferation was analyzed using CCK-8 and colony formation assay, together with an Annexin V-PE/7-AAD kit for cell apoptosis assay. Cell migration and invasion was detected using a Transwell migration and invasion assay. Methylation specific PCR was used to examine whether miR-410 promoter was demethylated. In this study, we validated that ERLIN2 was a direct target of miR-410 and miR-410 suppressed ERLIN2 expression at the post-transcriptional level. Importantly, the regulation of ERLIN2 by miR-410 was estrogen receptor (ER) dependent. ...Continue Reading

Citations

Oct 12, 2018·Journal of Leukocyte Biology·Fadoua BelaibaAmel Benammar Elgaaïed
Nov 30, 2018·Journal of Cellular Biochemistry·AmirReza HesariFaezeh Ghasemi
May 7, 2019·Acta Biochimica Et Biophysica Sinica·Yilin LinShaoqin Chen
Aug 15, 2019·The Journal of Obstetrics and Gynaecology Research·Yanyan ChenJiefang Zhou
Feb 11, 2020·BMC Genomics·Daria O KoninaMikhail Yu Skoblov
Jul 17, 2020·Non-coding RNA·Jun Sheng Wong, Yoke Kqueen Cheah
Dec 10, 2019·Clinical & Experimental Metastasis·Padacherri Vethil JishnuShama Prasada Kabekkodu
Jul 18, 2020·Cancer Biomarkers : Section a of Disease Markers·Xuan LiuYingliang Li
Jan 14, 2021·Nucleus·Norma E Padilla-MejiaMark C Field
Dec 4, 2020·Journal of Hematology & Oncology·Tianming ZhaoHui Zeng

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