miR-423-5p serves as a diagnostic indicator and inhibits the proliferation and invasion of ovarian cancer

Experimental and Therapeutic Medicine
Xuebiao TangNian Yang

Abstract

MicroRNA (miR)-423-5p is a potential target for the diagnosis and therapy of heart failure and cancer. The present study aimed to investigate the expression and role of miR-423-5p in ovarian cancer. miR-423-5p expression in ovarian tissues and plasma collected from ovarian cancer patients and healthy volunteers was analyzed by polymerase chain reaction analysis. In addition, a cell proliferation assay, clonogenic assay and Matrigel-based assay were performed to evaluate the role of miR-423-5p in ovarian cancer cells. The results demonstrated that miR-423-5p was downregulated in ovarian cancer tissues and plasma from ovarian cancer patients, compared with healthy individuals. Of note, miR-423-5p expression in ovarian tissues and plasma was demonstrated to be inversely correlated with ovarian cancer progression. Transfection with miR-423-5p efficiently increased miR-423-5p expression in A2780-s and A2780-cp cells, which had low miR-423-5p expression. Ectopic overexpression of miR-423-5p reduced cell proliferation, colony formation and invasion of ovarian cancer cells. In conclusion, the present study indicated that miR-423-5p may serve as a diagnostic indicator and functions as a tumor suppressor in ovarian cancer.

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Citations

Dec 22, 2019·Cells·Óscar Rapado-GonzálezMaría Mercedes Suárez-Cunqueiro
Jul 2, 2020·OncoTargets and Therapy·Ting DaiQing Gong
May 1, 2021·International Journal of Molecular Sciences·Jen-Tai Lin, Kuo-Wang Tsai

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